超量表达GRF9基因促进番茄生长并增强其对磷的吸收利用能力

GRF9 Over-expressing Improves Tomato Growth and Phosphorus Use Efficiency

研究了编码拟南芥14-3-3蛋白的基因grf9导入番茄并超量表达后,转grf9基因番茄在土壤不同供磷条件下的生长和对磷的利用特征。结果发现,在2个供磷水平下,转grf9基因番茄的地上部含磷量均显著高于野生型,且分别提高58.70%和56.68%,磷素累积量较野生型增加88%～90%;同时转grf9基因番茄的地上部干重较野生型增加幅度约20%。高供磷条件下种植转grf9基因番茄的土壤速效磷耗竭程度显著高于种植野生型番茄的土壤,亏缺度增加15.7%;相应地,高供磷条件下转基因番茄对土壤磷的农学利用率和生理利用率均有显著提高,分别增强达200%和165%。该结果为培育磷高效新品种提供了新思路和科学依据。

By generating and examining transgenic tomato over-expressing grf9 grown in a vegetable soil condition, this study aimed to investigate the effects of grf9 from arabidopsis on tomato growth and phosphorus （P） use efficiency. Results showed that in these two P-level conditions shoot P concentration increased significantly, improved by 58.70% and 56.68% respectively, P uptake increased by 88%-90%; while in the transgenic tomato, shoot dry weight increased by about 20%. Soil available P decreased significantly by about 15.7% planted the transgenic tomato in the additional P soil condition; accordingly over-expression of grf9 gene could improve P agronomic and physiological efficiencies in the additional P soil condition, and improved by 200% and 165% respectively. The results provided new idea and scientific basis for P use efficiency genetic modification.