白藜芦醇包合物的制备及其乳膏渗透性能考察

Preparation and Permeability Investigation of Resveratrol Hydroxypropyl β-Cyclodextrin Inclusion Compound

目的:制备白藜芦醇的羟丙基-β-环糊精(HP-β-CD)分子包合物,并考察不同促渗剂对该包合物乳膏渗透性能的影响。方法:采用冷冻干燥法制备白藜芦醇的HP-β-CD分子包合物,通过HPLC测定包合率。采用改良的Franz扩散装置,通过动物离体透皮吸收试验筛选最佳透皮吸收促渗剂。结果:白藜芦醇HP-β-CD包合物的包封率良好。促渗剂种类不同、加入量不同均能影响乳膏的渗透效果,最佳促渗剂为1%氮酮。结论:白藜芦醇制成包合物后稳定性良好,1%氮酮为促渗剂制成的白藜芦醇包合物乳膏具有良好的渗透能力,在美容方面具有较大的开发前景。

Objective： To prepare resveratrol hydroxypropyl-β-cyclodextrin（HP-β-CD） inclusion compound and investigate effect of 4 kinds of penetration enhancers on percutaneous absorption of this inclusion cream.Method： Resveratrol HP-β-CD inclusion compound was prepared with lyophilization technique,entrapment efficiency of this inclusion compound was investigated by HPLC.Modified Franz diffusion cell was applied to investigate in vitro transdermal absorption behavior of resveratrol HP-β-CD inclusion compound cream with isolated mouse skin as barrier and chose the best penetration enhancer.Result： Prepared resveratrol HP-β-CD inclusion compound was in high entrapment efficiency.There was large difference when different transdermal enhancers or different concentrations of enhancers were used,1% azone was optimum penetration enhancers.Conclusion： Resveratrol HP-β-CD inclusion compound had high stability,prepared cream with 1% azone as penetration enhancers had the best transdermal permeation behavior,resveratrol HP-β-CD inclusion compound cream had a great development prospect in the beauty.