茵栀黄分散片中3种活性成分的体外溶出度比较

Comparison of in vitro Dissolution of Three Active Components from Yinzhihuang Dispersible Tablets

目的:建立同时测定茵栀黄分散片中黄芩苷、绿原酸与栀子苷的体外溶出测定方法,并考察三者溶出特点。方法:以250 mL蒸馏水为溶出介质,采用桨法,转速50 r.min-1,通过HPLC测定茵栀黄分散片中绿原酸、栀子苷与黄芩苷的溶出度,计算累积溶出度,溶出曲线采用相似因子(f2)进行相似性比较。结果:以绿原酸为参比成分时,栀子苷与黄芩苷的f2分别为89.23,48.35;以栀子苷为参比成分,黄芩苷的f2=46.98。结论:以蒸馏水为溶出介质时,茵栀黄分散片中绿原酸与栀子苷具有相似的溶出特点,但与黄芩苷的溶出特点不同。

Objective： To establish an HPLC method for simultaneous determination of chlorogenic acid,jasminoidin and baicalin in Yinzhihuang dispersible tablets,and investigate in vitro dissolution characteristics of these three active components.Method： According to the 2010 edition of ‘Chinese Pharmacopoeia ’,with distilled water was dissolution medium,the paddle method was used with rotate speed of 50 r.min-1,the content of these active components were determined by HPLC,the accumulative dissolution was calculated and the resemblance of dissolution curve was compared by similarity factors（f2）.Result： Compared with chlorogenic acid,f2 of jasminoidin and baicalin were 89.23 and 48.35 in water,respectively;Compared with jasminoidin,f2 of baicalin was 46.98.Conclusion： Chlorogenic acid and jasminoidin from Yinzhihuang dispersible tablets had similar dissolution characteristics in distilled water,but baicalin was different from them.