UPLC-ESI-MS-MS分析生晒参和紫红参中皂苷类成分

Analysis on Ginsenosides of Dry Radix Ginseng and Purplish Red Ginseng with UPLC-MS-MS

目的:采用UPLC-ESI-MS-MS方法比较生晒参和紫红参中人参皂苷类成分的种类及含量,阐明紫红参特异成分。方法:Waters ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7μm)色谱柱,流动相A(0.1%甲酸)-B(乙腈)梯度洗脱,柱温30℃;质谱条件:电喷雾离子化源(ESI),负离子检测模式。结果:与生晒参相比,紫红参中人参皂苷的种类和数量发生了改变,检测出含量较高的人参皂苷为20(S)-人参皂苷Rg3、20(R)-人参皂苷Rg3、20(R)-人参皂苷Rh1、20(R)-人参皂苷Rg2,Rk1,Rg5,Rd,Ro、姜状三七苷R1等。结论:人参加工成紫红参后皂苷类成分发生较大的变化,产生了大量稀有皂苷,人参加工可能产生紫红参的特异成分。

Objective： To clarify special constituents of purplish red ginseng,the kinds and content of ginsenosides in dry radix ginseng and purplish red ginseng were determined by UPLC-ESI-MS-MS method in this paper.Method： Waters ACQUITY UPLC BEH C18（2.1 mm × 50 mm,1.7 μm） column was used with mobile phase consisted of 0.1% methanoic acid-acetonitrile（gradient elution）.The column temperature was set at 30 ℃.MS conditions： ESI,the negative electrospray ionization mode.Result： Compared with the dry radix ginseng,the kinds and content of ginsenosides in purplish red ginseng had changed,the content of 20（S）-ginsenoside Rg3,20（R）-ginsenoside Rg3,20（R）-ginsenoside Rh1,20（R）-ginsenoside Rg2,Rk1,Rg5,Rd,Ro and zingibroside R1 increased in purplish red ginseng.Conclusion： Great changes of saponins have been obviously found in processing ginseng to purplish red ginseng.Special constituents of purplish red ginseng could be produced in ginseng processing.