百咳静糖浆质量标准

Quality Standard of Baikejing Syrup

目的:建立百咳静糖浆的质量控制标准。方法:采用TLC对黄芩、黄柏、陈皮、麻黄进行定性分析,HPLC测定百咳静糖浆中黄芩苷含量。结果:百咳静糖浆供试品与对照品色谱的相应位置上,显相同颜色斑点;Sinochrom ODS bp C18色谱柱(4.6 mm×250 mm,5μm),ODS预柱,流动相甲醇-水-磷酸(45∶55∶0.04),检测波长315 nm,黄芩苷在0.08～0.4μg呈良好线性关系(r=0.999 8),平均回收率100.9%(RSD 1.7%)。结论:该方法灵敏、准确可靠、重复性好,可用于百咳静糖浆的质量控制。

Objective： To establish qualitative control standard of Baikejing syrup.Method： TLC was used for qualitative analysis of Scutellaria baicalensis,Phellodendron chinense,Citrus reticulata,Ephedra sinica,the content of baicalin was determined by HPLC.Result： Baikejing syrup for samples and reference substances showed the same color dot in corresponding chromatograph position;Sinochrom ODS bp C18 column（4.6 mm × 250 mm,5 μm） and ODS pre-column were adopted with mobile phase of methanol-water-phosphoric acid（45 ∶ 55∶ 0.04）,detection wavelength was at 315 nm,baicalin showed a good linear relationship within the range of 0.08-0.4 μg（r = 0.999 8）,the average recovery was 100.9% with RSD of 1.7%.Conclusion： This method was sensitive,reproducible and reliable,it could be used for quality control of Baikejing syrup.