紫菀丸对血热出血大鼠血液流变性、凝血时间及肺部的影响

Effects of Ziwan Wan on Hemorheological Parameters,Clotting Time and Lung Presentations in Rats with Fevered and Bleeding

目的:考察紫菀丸对血热出血模型大鼠血液流变性、凝血时间及肺部的影响。方法:SD大鼠随机分为6组,分别为空白对照组,模型对照组,云南白药组(0.5 g.kg-1),紫菀丸高、中、低(5,2.5,1.25 g.kg-1)剂量组。将供试品按每日20 mL.kg-1的剂量灌胃(ig),2次/d,给药7 d。除空白对照组外,其余组复制血热出血大鼠模型,并比较紫菀丸对其血液流变性、凝血酶时间(TT)、活化部分凝血酶原时间(APTT)、凝血酶原时间(PT)、纤维蛋白含量(FIB)以及肺部的影响。结果:模型对照组较空白对照组有显著性差异,明显升高全血黏度,血浆黏度,TT,APTT明显延长,PT显著缩短,FIB含量显著增加。紫菀丸中剂量组能够显著降低血热出血模型大鼠的全血黏度,血浆黏度,APTT,TT,明显增加FIB的含量。紫菀丸高剂量组能明显缩短TT,紫菀丸低剂量组能显著延长PT。紫菀丸中剂量组依次为全血黏度200 s-1(5.87±0.24)mPa.s,30 s-1(7.60±0.36)mPa.s,5 s-1(13.70±0.51)mPa.s,1 s-1(32.38±2.06)mPa.s,APTT(17.11±0.78)s,TT(30.07±2.00)s,FIB(5.56±0.14)g.L-1;紫菀丸高剂量组为TT(30.74±2.96)s;紫菀丸低剂量组为PT(14.63±1.14)s;与模型对照组比较P<0.05或P<0.01。在肺体征方面,紫菀丸能够改善血热出血大鼠肺部损伤的相关症状,中剂量效果最好,其次是高、低剂量。结论:紫菀丸中剂量组能够改善血热出血大鼠异常的血液流变学指标,通过影响内、外源性凝血酶及纤维蛋白原来达到止、凝血效果,紫菀丸高剂量组对部分血液流变学和凝血指标有一定的效果。紫菀丸剂量组对模型组大鼠肺部的损伤都有一定的治疗作用。

Objective： To investigate the effects of Ziwan Wan on the hemorheological parameters,clotting time and lung presentations in rat fevered and bleeding model.Method： The SD rats were divided into blank control group,model compared group,Yunnan Baiyao 0.5 g.kg-1 group,Ziwan Wan（high,middle and low,5,2.5,1.25 g.kg-1 accordingly） dose group.The corresponding drugs were iggiven with dose of 20 mL.kg-1 twice a day,for 7 days.Except the blank control group,fevered and bleeding models were established.The hemorheological parameters,thrombin time（TT）,activated partial thromboplastin time（APTT）,the prothrombinase time（PT）,fibrinogen（FIB） and the signs of lung presentations were detected in these groups.Result： In the model group compared with blank control group,of whole blood viscosity and plasma viscosity were increased,TT,APTT and FIB were prolonged,PT significantly was shorten.The middle dose of Ziwan Wan could dramatically lower whole blood viscosity,plasma viscosity,APTT,TT and increase the content of FIB.The high dose of Ziwan Wan could significantly shorten the TT,Low dose of Ziwan Wan could significantly prolong PT.The whole blood viscosity,APTT,TT and FIB in the middle dose of Ziwan Wan group was respectively 200 s-1（5.50 ±0.15） mPa.s,30 s-1（7.64 ±0.19） mPa.s,5 s-1（13.70 ± 0.51） mPa.s,1 s-1（32.38 ± 2.06） mPa.s,APTT（17.11 ± 0.78） s,TT（30.07 ± 2.00） s,FIB（5.56 ± 0.14） g.L-1;TT of high dose of Ziwan Wan group was that TT（30.74 ± 2.96） s;PT of low dose of Ziwan Wan group was that PT（14.63 ± 1.14） s,compared with the model group,P〈0.05 or P〈0.01.Ziwan Wan could improve lung injury related symptoms in fevered and bleeding model groups,The middle dosage showed the best effect.Conclusion： The middle dose of Ziwan Wan showed the effect of Cooling blood and hemostasis by ameliorating the abnormal hemorheological parameters.The middle dose of Ziwan Wan is effective for hemostasis,and plays roles by ways of endogenous thrombin,extrinsic thombin and fibrin.The high dose of Ziwan Wan has certain effect for part of the abnormal hemorheological parameters and hemostasis parameters.Three doses of Ziwan Wan showed therapeutical effect in damaged lungs in fevered and bleeding model.