动脉粥样硬化秽浊痰阻证动物模型的研究

Study on Establishing Animal Model of Huizhuo Tanzu Type Atherosclerosis

目的:建立动脉粥样硬化秽浊痰阻证发病特点相符合的病证结合动物模型,为后续的临床研究提供载体。方法:将60只载脂蛋白E基因敲除小鼠随机分为4组,即高脂饲料复合寒燥环境的模型组、高脂饲料组、寒燥环境组和正常对照组。过程中记录小鼠体重、情绪、大小便、毛发及舌象等生物表征,12周时眼球取血,用酶比色法检测血清甘油三酯、总胆固醇,高密度脂蛋白,低密度脂蛋白;脱臼处死小鼠,分离主动脉瓣至髂肾动脉分支动脉组织,观察细胞形态学变化。用SPSS17.0对量化后指标进行统计分析。结果:高脂饮食复合寒燥环境组小鼠表现为行动迟缓,抑郁状态,进食进水较少,体重增长不明显,舌质多见暗红或暗紫色;模型组小鼠主动脉中膜明显增生,平滑肌细胞排列紊乱并向内膜突起,大量粥样斑块形成,管腔明显狭窄,可见少量泡沫细胞,大量胆固醇结晶形成;高胆固醇饲料和寒燥环境对ApoE基因敲除小鼠的血脂升高的促进作用不显著。结论:高脂饲料喂养复合寒燥环境干预建立动脉粥样硬化秽浊痰阻证的方法是可行的,可成功建立与动脉粥样硬化秽浊痰阻证生物表征和部分生物学基础相似的病证结合模型。

Objective： Study the way to make Huizhuo Tanzu type atherosclerosis animal model.Method： Divide 60 ApoE gene knock out mouse into 4 groups： high fat diet and cold dry environment intervention animal model group,high fat diet control group,cold dry environment intervention control group and normal control group.12 weeks after nurturing in different groups,the weight,emotion,defecation,tongue presentations were observed in all animals.Biochemical indicators and cell morphology were compared between groups after sacrificing all the animals.Result： Indicators of Huizhuo Tanzu type atherosclerosis in High fat diet and cold dry environment intervention animal model group animals are significantly higher than other groups（P〈0.05）.Light microscopic observations revealed that all the animals developed atheromatous plaque.high fat diet and cold dry environment intervention alone has significantly different affect on the development of atheromatous plaque comparing to a combined intervention of both high fat diet and cold dry environment（ANOVA,P〈0.05）.Conclusion： Animal underwent combined intervention of high fat diet and cold dry environment significant biological characteristics of Huizhuo Tanzu type atherosclerosis,indicating that animal models were successfully made.