茜草和茜草炭对正常大鼠凝血-纤溶系统的影响

Effects of Rubia cordifolia and Charred R. cordifolia on Coagulation-fibrinolytic System of Normal Rats

目的:研究茜草和茜草炭对正常Wistar大鼠凝血-纤溶系统的影响,通过其药理作用探讨它们的止血化瘀机制。方法:将Wistar大鼠随机分为6组,每组7只,分别为茜草和茜草炭水煎制低剂量、高剂量组(含生药5.0,10.0 g.kg-1),阳性对照组(氨甲苯酸0.6 g.kg-1),空白对照组(生理盐水10 mL.kg-1)。灌胃7 d,用ELISA法检测大鼠血浆中组织型纤溶酶原激活剂(t-PA)、纤溶酶原激活物抑制剂(PAI)和纤溶酶原(PLG)活性。结果:和空白对照组比较,茜草在实验剂量下均能提高t-PA的活性,且高剂量时提高t-PA的活性更显著(P<0.01)。高剂量茜草炭能使大鼠t-PA活性提高(P<0.05),但其作用低于高剂量茜草组。低剂量时对大鼠t-PA活性影响不显著。茜草和茜草炭对PAI和PLG的活性影响均不显著。结论:茜草高、低剂量均能提高t-PA的活性,且在高剂量时提高更显著,进而影响凝血-纤溶系统,从而发挥其止血化瘀作用。茜草炭对纤溶系统也有一定作用,但可能是多种酶的综合作用。茜草和茜草炭对凝血-纤溶系统药理作用效果不同,进而影响它们的临床使用。

Objective： To study the effects of Rubia cordifolia L.and Charred R.cordifolia on coagulation-fibrinolytic system in normal Wistar rats and their mechanism of stasis-resolving hemostatic based on their pharmacological action.Method： Wistar rats were randomly divided into blank control group（normal saline,10 mL.kg-1）,R.cordifolia low-dose（5.0 g.kg-1） and high-dose（10.0 g.kg-1） groups,Charred R.cordifolia low-dose（5.0 g.kg-1） and high-dose（10.0 g.kg-1） groups and positive control group p-aminomethylbenzoic acid,（0.6 g.kg-1） ig,one time a day for 7 days.The blood in abdominal aorta was taken and then the plasma was separated in the eighth day.The activities of plasma tissue-type plasminogen activator（t-PA）,plasminogen activator inhibitor（PAI） and plasminogen（PLG） in plasma were measured by ELISA method.Result： Compared with the blank control group,both the low-dose and high-dose group of R.cordifolia can increase t-PA activity,the high-dose group of R.cordifolia can increase t-PA activity more obviously（P〈0.01）.The high-dose group of Charred Rubia cordifolia L.can also increase t-PA activity（P〈0.05） but the low-dose group of Charred Rubia cordifolia L.cannot.In addition,the high-dose group of Charred R.cordifolia＇s effect on t-PA activity is smaller than the high-dose group of R.cordifolia The effects of R.cordifolia and Charred R.cordifolia on the PAI and PLG activity are all not significant regardless of their dosage.Conclusion： Both the low-dose and the high-dose group of R.cordifolia can remarkably affect fibrinolytic system to play its role on stasis-resolving hemostatic through increasing t-PA activity.Charred R.cordifolia can also affect fibrinolytic system while it may be the comprehensive effects of many enzymes.R.cordifolia and Charred R.cordifolia have different pharmacological effects on fibrinolytic system.Thereby,they have different uses in clinical practice.