日本北海道特产小果树HASKAOP生根组培苗移栽技术的研究

STUDY ON TRANSPLANTING TECHNOLOGY OF TISSUE CULTURE SEEDLING OF HASKAOP

本试验以苗龄为65d的日本北海道特产小果树HASKAOP带根组培苗为材料,研究不同栽培基质、炼苗方式、移栽季节,以及使用不同激素对小果树移栽成活的影响。结果表明:最适合小果树的栽培基质是蛭石:草炭=1:1,成活率为59.0%,新叶数可达3.34片,且植株健壮、叶色正常;采用炼苗方式三时,小果树组培苗的成活率最大为65.0%,比不经炼苗成活率提高52.0%;春季是最适宜小果树组培苗的移栽季节,成活率达62.0%;移栽前使用100mg/L ABT的成活率比空白对照组仅提高5.3%,且其他种类激素的使用对提高小果树组培苗成活率无明显效果。

Using the tissue culture with root aged for 65 days of HASKAOP as test materials in multi - factor orthogonal experiment, the purpose was to analyse the effect of different cultivation medium, methods of hardening plantlets and seasons on transplanting of tIASKAOP , as well as the survival percent after the treatments of different hormones on transplanting of ttASKAOP. The results showed that 59.0% survival percent was obtained in the cuhivation medium of base soil with vermiculite ： peat soil = 1 ： 1 and in good growth condition, the average leaf blade increased 3.34 pieces. The survival percent in the third way of hardening plantlets was 65.0%, higher than other methods and 53.0% higher than the ck. The optimal time for transplanting of HASKAOP was spring and the survival percent was 62.0%. The survival percent of using100mg/L ABT before transplanting was 5.3% higher than the ck, but the transplanting effi- ciency of HASKAOP had barely improved with using other hormones.