流行性出血热患者尿蛋白定量及SDS-PAGE检测的临床意义

Clinical significance of quantitative analysis and SDS - PAGE of proteinuria in patients with epidemic hemorrhagic fever

作者对102例流行性出血热(EHF)患者行尿蛋白定量及尿蛋白^(12)烷基硫酸钠-聚内烯酰胺凝胶电泳(SDS-PAGE)检测.结果表明:EHF轻中型、重型、危重型分别以轻度、中度、重度蛋白尿为主.本病各型与各期均可排出混合性蛋白尿,但以危重型及极期更为突出(P<0.05-0.01).蛋白尿中以反映肾小球损伤的中分子量尿蛋白排出量最大,持续时间最长(P<0.05).在69.6％尿蛋白转阴例中SDS-PAGE仍有29.2％蛋白组分异常.同时检测尿蛋白含量与SDS-PAGE可提示EHF肾实质损害的程度及部位,并为观察其病情演变、判断预后、研究肾衰发病机理等方面提供又一指标.

The quantitative analysis and sodium dodccyl sulfalc polyacylamidc gel clcctrophoresis ( SDS - PAGF ) of protcinuria were performed in 102 paticnts with epidemic hemorrhage fever (FHF ). The results showed that the mild protcinuria was chiefly in mild or moderate types of F.HF patients . while moderate prolcinuria in severe type and the evident prolcinuria in crisis type . Although the mixed protcinuria appeared in each type and each phase of the illness , it increased greatly in severe types and crisis phases . It was found thai excretion of middle molecular weight protein in urine that reflected the glomcrular damage was in the greatest amount and longest duration . Protcinuria in some patients (69.6% ) turned negative , however. SDS - PAGF showed that 29.2% protein components remained abnormal . The quantitative analysis and SDS - PAGF not only reflect the severity and cxtcnsivcncss of the damage of renal parenchyma in FHF bul also provide a new indicator for evaluating the variation and prognosis of the disease and studying the mechanism of rcnal failure in FHF