胶质瘤碱性蛋白iRNA激活的小鼠脾细胞中c-myc,IL-2和IFN-γ基因的表达

Dynamic expression of c - myc, IL - 2 and IFN- γgenes of mouse splenocytes activated by glioma base protein specific iRNA

作者采用分子杂交技术观察了经胶质瘤碱性蛋白iRNA激活的小鼠脾淋巴细胞中c-myc,IL-2和IFN-γ基因mRNA水平的动态变化.结果表明,iRNA能在相应抗原存在下激活小鼠脾淋巴细胞表达c-myc,IL-2和IFN-γ基因;c-myc基因在细胞刺激后3-6 h表达最高.IL-2和IFN-γ基因在细胞刺激后12 h表达最高.

Dot blotting hybridixation technique was used to observe the dynamic expression of c -myc,IL- 2 and IEN - γgenes in mouse splcnocytcs, activated by glioma base proton (GBP ) - specific iRNA . The result showed thai iRNA induced mouse splcocyles, to express c - myc . IL - 2 and U-N - γ. genes in the presence of corresponding antigens , the maximal expression of c - myc gene appeared in 3 - 6 hours after iRNA added to the cell culture , while expression of 1L - 2 and IFN - 7 genes occurred in about 12 hours