不同部位诱发癫痫动物模型对比研究

Comparative Study of epileptic animal models induced by different site

目的探讨铁离子不同部位诱发外伤性癫痫动物模型的稳定性和可靠性。方法健康成年雄性大鼠50只,随机分为5组:SDA、SDB、SDC、SDD、SDE,各10只,其中SDA、SDB为对照组;SDC、SDD、SDE为癫痫模型组,采用不同方法制备癫痫模型,点燃成功后,收集行为表现、脑电图、组织病理等3方面资料,行统计学分析。结果研究结果显示SDC、SDD、SDE均能点燃癫痫动物模型,其中SDD点燃动物模型出现早,持续时间短,不适合作为人类癫痫基础研究;组织病理方面,SDA和SDB比较P>0.05,差异无统计学意义,排除实验干扰导致海马CA1神经元细胞丢失;SDC、SDD、SDE均能使CA1区锥体细胞数减少,与对照组相比P<0.01,差异有统计学意义;对侧相比P<0.01,差异有统计学意义;3组同侧之间比较P>0.05,差异无统计学意义。结论额叶皮层运动区注射铁离子操作简单、成功率高、可靠性好,相比于其他两种,更为适合外伤性癫痫基础研究和药物研究。

Objective To explore the different position induced posttraumatic epilepsy animal model stability and reliability.Methods 50 healthy adult male rats were randomly divided into 5 groups：SDA,SDB,SDC,SDD,SDE,10,SDA,SDB as the control group,SDC,SDD,SDE as epileptic model group,were prepared by different methods after successful epilepsy model was lighted respectively.Behavior,electroencephalogram,and histopathological observation of three aspects,data collection,statistical analysis were performed.Results The study indicates that the SDC,SDD,SDE kindled epilepsy animal model.SDD lighted animal model appeared early,short duration,was not suitable as a human epilepsy foundation research;The difference of tissue pathology,SDA and SDB comparison was not statistically significant P0.05,exclusion experiment interference resulting in hippocampal CA1 neurons cell loss;SDC,SDD,SDE decreased the CA1 pyramidal cell number,compared with the control group P0.01,the differences was significant compared with the contralateral,P0.01,the comparison of the three groups of ipsilateral has no significance.Conclusion Prefrontal cortex motor area injection of iron ion has the advantages of simple operation,high success rate,good reliability,compared to the other two,is more suitable for traumatic epilepsy in basic research and drug research.