川芎嗪注射液对铅中毒小鼠的治疗作用及对海马NO和nNOS的影响

Therapeutical effects of ligustrazine hydrochloride injection on lead poisoning mice and the influence on NO and nNOS of hippocampus

目的研究川芎嗪注射液对铅中毒小鼠学习记忆功能的影响,并初步探讨其作用机制。方法将50只小鼠随机分成5组:空白对照组、模型对照组、阳性对照组、川芎嗪注射液低剂量组和川芎嗪注射液高剂量组。用小鼠跳台、水迷宫检测小鼠学习记忆功能,微分电位溶出法测定血铅和脑铅含量,硝酸还原酶法检测NO含量;免疫组织化学方法观察nNOS阳性细胞表达。结果川芎嗪注射液高剂量组能显著改善铅中毒小鼠的学习记忆障碍。与模型对照组相比,川芎嗪注射液高剂量组能显著降低血铅和脑铅浓度,增高海马NO含量,且增加nNOS阳性神经元表达。结论川芎嗪注射液能改善铅中毒小鼠的学习记忆功能,其作用机制可能与海马NO含量增高及nNOS神经元表达增高有关。

【Objectives】 To study the effects of learning and memory on lead poisoning mice treated by ligustrazine hydrochloride injection and to explore the mechanism preliminarily.【Methods】 The 50 mice were randomly divided into five groups： blank group,negative control group,positive control group,ligustrazine low dose group and high dose group.Step-down test and Morris water maze were performed to evaluate the learning and memory ability of mice.The lead concentrations of blood and brain were measured by electric potential stripping method.In the mean time,the content of NO was tested by nitrate reductase method.The expression of nNOS positive cells was observed by immunohistochemistry methods.【Results】 Compared with that of negative control group,the learning and memory ability of lead poisoning mice was markedly improved in ligustrazine high dose group,and the lead concentrations of blood and brain were significantly decreased,the hippocampal intracellular NO content was obviously increased,and the nNOS positive cells in the CA1 and CA3 region of hippocampus were significantly increased.【Conclusions】 Ligustrazine hydrochloride injection can improve the learning and memory on lead poisoning mice.The mechanism may be related with in-creasing NO content and the expression of nNOS positive cells in hippocampus.