新疆维吾尔族、汉族婴幼儿毛细支气管炎与β_2-AR基因多态性的相关性研究

Relationship between genetic polymorphisms of β_2-adrenergic receptor and infants bronchiolitis of Han and Uighur in Xinjiang

目的了解维吾尔族、汉族婴幼儿毛细支气管炎与β2-肾上腺素能受体(β2-AR)基因之间的关系。方法随机抽取毛细支气管炎患儿131例、正常儿童110例进行病例对照研究,采用PCR扩增β2肾上腺素能受体基因,以DNA直接测序法对目的基因多态性进行分析,采用BLAST将测序结果与NCBI基因库进行相似性序列比较。结果①维吾尔族毛细支气管炎组与对照组β2-AR16基因型和等位基因频率分布差异均有统计学意义(P<0.05);汉族中β2-AR16基因型分布差异无统计学意义,但等位基因频率分布差异却有统计学意义(P<0.05)。②维吾尔族、汉族的β2-AR27基因型分布差异无统计学意义,但等位基因频率分布差异具有统计学意义(P<0.05);汉族病例组与对照组间β2-AR27基因型和等位基因频率分布差异均有统计学意义(P<0.05)。结论新疆维、汉两个不同民族毛细支气管炎患者β2-AR基因多态性表现出明显的族别差异。

【Objective】 To investigate the relationship between genetic polymorphisms of β2-adrenergic receptor and infants bronchiolitis.【Methods】 The total DNA form of the infants with bronchiolitis（n =131） and normal infants（n =110） were investigated,β2-adrenergic receptor gene was amplified by PCR and sequenced by DNA sequencing technology,the similarity of sequencing results and NCBI gene bank were compared by BLAST.【Results】 The genotype distribution of β2-adrenergic receptor gene at both β2-AR 16 and β2-AR 27 loci in the two groups were statistically significantly different（P 0.05）.There was no significant difference in the frequencies of genotypes of β2-AR 27 loci between the two groups（P 0.05）,there was significant difference in the frequencies of alleles of β2-AR 27 loci between the two groups.The genetic polymorphisms of β2-adrenergic receptor in the infant of males and females demonstrated no statistical difference（P 0.05）.【Conclusion】 Genetic polymorphisms of β2-adrenergic receptor is associated with infants bronchiolitis.