原发性高血压高迁移率族蛋白1与血管内皮功能的关系

Relationship between HMGB 1 and vascular endothelial function in essential hypertension

目的探讨原发性高血压(EH)患者血清高迁移率族蛋白1(HMGB1)与血管内皮功能的关系。方法连续收集高血压患者30例,正常对照组30例。分别采用酶联免疫吸附法、高效液相色谱法、硝酸还原酶法测定血清HMGB1、血浆非对称性二甲基精氨酸(ADMA)、血清一氧化氮(NO)水平,同时采用高分辨超声检测肱动脉血流介导的内皮依赖性血管舒张功能(FMD)。结果高血压组血清HMGB1、血浆ADMA显著高于对照组(P<0.01),血清NO、FMD显著低于对照组(P<0.01)。Spearman秩相关分析显示,在所有研究对象中,HMGB1与ADMA呈正相关(r=0.757,P<0.01),与NO、FMD呈负相关(r=-0.743,P<0.01;r=-0.707,P<0.01)。以FMD为应变量进行多元线性逐步回归分析,HMGB1是FMD的独立影响因子之一。结论血清炎症因子HMGB1可能参与了高血压血管内皮功能损伤过程。

【Objective】 To explore whether serum high mobility group box 1（HMGB1） is associated with vascular endothelial function in essential hypertension.【Methods】 Serum HMGB1,plasma asymmetric dimethylarginine（ADMA）,serum nitric oxide（NO） and brachial artery flow-mediated vasodilation（FMD） were determined by enzyme-linked immunosorbent assays（ELISA）,high performance liquid chromatography（HPLC）,nitrate reductase method and high-frequency ultrasonography,respectively,in 30 essential hypertensives and 30 matched healthy control subjects.【Results】 Serum HMGB1 level and plasma ADMA level in essential hypertension group were higher than that in controls（P 0.01）.Meanwhile,essential hypertensives had lower FMD and serum NO than controls（P 0.01）.Spearman rank correlation analysis indicates that in all subjects,HMGB1 was positively correlated with ADMA and negatively correlated with NO and FMD（r =0.757,P 0.01;r =-0.743,P 0.01;r =-0.707,P 0.01,respectively）.HMGB1 was one of the independent predictors of FMD in multiple linear regression models.【Conclusion】 HMGB1 as a serum inflammatory factor may be involved in the impaired endothelial function in essential hypertension.