表型确认金属β内酰胺酶的四种方法的比较

An evaluation of four different phenotypic techniques for detection of metallo-β-lactamase

目的评价表型确认金属-β-内酰胺酶的四种方法,寻求适合实验室常规开展的表型确认方法。方法分别用改良Hodge试验、纸片扩散法、E-test纸条检测法和双纸片协同试验检测临床分离的7株耐亚胺培南肺炎克雷伯菌的金属-β-内酰胺酶产酶情况,并用PCR方法进行确认。同时对这四种方法进行了方法学评价。结果四种方法均能检出金属-β-内酰胺酶。结论 E-test纸条检测法是临床实验室表型确认金属酶的最好方法。改良Hodge试验是检测金属-β-内酰胺酶的初筛试验。

【Objective】The present study was undertaken to detect metallo-β-lactamase（MBL） in isolates of Klebsiella Pneumoniae by four different phenotypic methods,in order to find a phenotypic method which can be used as a convenient method in the clinical laboratory.【Methods】Seven isolates of Klebsiella Pneumoniae were treated with modified Hodge test,disk diffusion method,E-test strip for detection method and double disk synergy test to detect Metal-β-lactamase production,and the four methods were evaluated by contrasting test of PCR.【Results】 Metallo-β-lactamase was detected by four different methods.【Conclusion】E-test strip for detection metal-β-lactamas is the best phenotyptic confirming test of clinical laboratory method.Modified Hodge test is the screening test for detecting metal-β-lactamase.