硫化氢后适应对大鼠心肌缺血-再灌注损伤后心功能的影响

Hydrogen sulfide postconditioning on myocardial ischemia-reperfusion injury in cardiac function

目的探讨硫化氢后适应对大鼠心肌缺血-再灌注损伤的保护作用。方法采用结扎大鼠左冠状动脉前降支30 min,再灌60-120 min的方法制备在体心肌缺血-再灌注损伤模型。Weistar大鼠（n=24）随机分为3组（n=8）：假手术组（Sham）,缺血再灌注组（I/R）和H2S后适应组（H2S）。Sham组为仅穿线,不做结扎;I/R组为先缺血30 min,再灌120 min;H2S后适应组为缺血30 min后,在再灌注之前经股静脉注射NaHS（15μmol/kg）。记录缺血及再灌注时心率、再灌注性心律失常、左室收缩末期压（LVESP）及左室舒张末期压（LVEDP）及左室内压上升/下降最大速率（±dp/dtmax,mmHg/s）等心功能参数,并计算左室发展压（LVDP,LVDP=LVESP-LVEDP）。应用伊文氏蓝和TTC染色测定心肌梗死面积（%）。结果 H2S后适应组与I/R组降低了再灌注室性心律失常的发生率、持续时间及心律失常评分（P〈0.05）,使心肌梗死面积减小（P〈0.05）;同时,心功能参数得到明显改善（P〈0.01）。结论 H2S后适应能够减轻大鼠心肌缺血再灌注损伤,其中在拮抗再灌注性心律失常和恢复大鼠心功能方面作用明显。

【Objective】 To evaluate the effect of hydrogen sulfide postconditioning on myocardial ischemia-reperfusion injury in rats.【Methods】 The left anterior descending coronary artery was ligated for 30 min followed by a 180 min reperfusion in vivo.Wistar rats（n =24） were randomly divided into 3 groups（n =8）.The sham group（Sham） was subjected to only threading but no ligation,ischemia-reperfusion group（I/R） to ischemia for 30 min and reperfusion for 120 min,and H2S postconditioning group（H2S） to 30 min of ischemia,then reperfused with NaHS（15 μmol/kg）.Heart rate,reperfusion arrhythmias,left ventricular end-systolic pressure（LVESP）,left ventricular end-diastolic pressure（LVEDP）,left ventricular pressure rise/fall maximum rate（±dp/dtmax,mmHg/s） and left ventricular developed pressure（LVDP,LVDP=LVESP-LVEDP） were recorded and calculated.The myocardial infarct size（%） was measured by the application of Evans blue and TTC staining.【Results】 Incidence of ventricular fibrillation,its duration,arrhythmia score and myocardial infarct size were significantly lower in the H2S postconditioning group and I/R group（P 0.05）,while the cardiac function parameters was improved significantly（P 0.01）.【Conclusion】 H2S postconditioning could significantly reduce myocardial ischemia-reperfusion injury.