基于均匀设计优化的大螟实用饲料配方及继代饲养

An Oligidic Diet for Sesamia inferens Optimized by Uniform Design and Successive Rearing

通过均匀设计对大螟人工饲料配方进行了优化,获得的实用饲料优化配方如下:琼脂3g,大豆粉8g,茭白粉11g,酵母16g,蔗糖6g,稻茎粉3g,玉米粉11g,麦芽粉5g,干酪素7g,维生素C 0.8g,山梨酸0.64g,金霉素0.54g,胆固醇0.04g,无机盐1.5g,蒸馏水200g。该配方对大螟已连续饲养超过10个世代,优于以往报道的饲料。该配方饲养的大螟第1代初孵化幼虫发育至蛹的比例(化蛹率87.5%)较原配方(CK,40.8%)提高46.7个百分点。从第1代到第10代的连续饲养过程中,大螟的化蛹率、羽化率、卵孵化率和单雌产卵量等指标没有明显退化趋势,平均分别为42.5%～87.5%、79.7%～95.8%、90.0～94.9%和95～252粒/雌。第10代除雄幼虫历期显著较长外,其余各指标均与第1代无显著差异。

An oligidic diet for larvae rearing of the pink stem borer（PSB）,Sesamia inferens（Walker）,was optimized by using the uniform design.The formulation of the optimized diet was as follows： agar powder 3 g,soybean powder 8 g,water bamboo powder 11 g,yeast powder 16 g,sucrose 6 g,rice powder 3 g,corn powder 11 g,wheat germ powder 5 g,casein 7 g,vitamin C 0.8 g,sorbic acid 0.64 g,aureomycin 0.54 g,cholesterol 0.04 g,Wesson＇s salt 1.5 g,and distilled water 200 g.PSB had been reared on the diet for more than ten successive genenrations,much more than that previously reported.The rate of the 1st instar PSB larvae that developed to pupae on the optimized diet reached 87.5%,significantly higher than that on the benchmark diet.As for the ten generations＇ succesive rearing,the average of pupation rate,emergence rate,egg hatching rate and fecundity of PSB on the optimized diet was 42.5%-87.5%,79.7%-95.8%,90.0%-94.9% and 95-252 eggs per female,respectively.There was no significant difference between the 10th genenation and the 1st of PSB reared on the diet,except the significantly longer male larval stage.