摘要
目的研究内皮细胞分泌的基质细胞衍生因子1(SDF-1)是否影响CXCR4+干细胞迁移功能。方法从骨髓中分离出CXCR4+的骨髓间充质干细胞(CXCR4+BMSC),用氧化型低密度脂蛋白(ox-LDL)刺激人脐静脉内皮细胞(HUVEC),用MTT法检测HUVEC细胞增殖,用RT-PCR检测SDF-1αmRNA的表达,用ELISA检测SDF-1α蛋白表达,用Transwell检测CXCR4+BMSC迁移。结果 ox-LDL的刺激影响HUVEC增殖,并引起SDF-1αmRNA和蛋白表达升高;含SDF-1α培养基上清能促进CXCR4+BMSC迁移,这种迁移可被CXCR4抗体抑制。结论 CXCR4+BMSC可通过SDF-1α/CXCR4轴向引起内皮细胞发生迁移运动。
Objective To study whether the stromal cell-derived factor 1 （SDF-1） secreted by endothelial cells affects the CXCPA^＋ stem cell migration. Methods CXCR4^＋ bone marrow-derived mesenchymal stem cells （BMSCs） were isolated from mouse bone marrow. Human umbilical vein endothelial cells （HUVECs） were stimulated with oxidized low-density lipoprotein （ox-LDL）, and the cell proliferation was detected by MTT assay, the expressions of SDF-1 α m RNA and protein were detec-ted by RT-PCR and ELISA, respectively. The migration of CXCR4^＋ BMSCs was analyzed by Transwell chamber assay. Results The stimulation of ox-LDL affected the proliferation and increased significantly the expression levels of SDF-1α mRNA and protein in HUVECs, and the medium supernatant promoted the migratory response of CXCR4^ ＋ BMSCs. When the neutralizing CXCR4 antibody eliminated the secreted SDF-1α, the migratory activity markedly decreased. Conclusion CXCR4^＋ BMSCs might migrate to endothelial cells by SDF-1α/CXCR4 axis in the atherosclerosis process.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2013年第8期805-808,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
湖北省教育厅项目(Q20122805)
湖北科技学院项目(ZX1125)
