摘要
目的探讨caspase募集结构域家族成员18(CARD18)在凋亡素诱导胃癌细胞凋亡的机制及在胃癌发生发展中的作用。方法运用二维凝胶电泳法分离凋亡素作用胃癌细胞后表达的差异蛋白质,通过基质辅助激光解吸附飞行时间质谱技术(MALDI-TOF-MS)和生物信息学方法确定其中部分差异蛋白质,RT-PCR和Western blot法检测CARD18的表达,采用免疫组化检测56例胃癌组织及癌旁组织中CARD18的表达,并分析其与胃癌临床病理参数的关系。结果凋亡素处理后的胃癌细胞CARD18表达下调最明显,CARD18蛋白在胃癌组织中的表达明显高于癌旁组织(P<0.05),且与胃癌的分化程度和淋巴结转移有关(P<0.05)。结论 CARD18可作为一个潜在的预后标志物和靶向标志物。
Objective To study the expression of caspase recruitment domain family member 18 (CARD18) in apoptin-induced apoptosis of gastric cancer cells and its role in the development of gastric cancer. Methods After gastric cancer cells were transfected with apoptin, differentially expressed proteins between the apoptin-expression SGC7901 group and the control group were seperated using two-dimensional gel electrophoresis and identified using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and Mascot database (http: //www. matrixscience, corn/). The expression level and location of CARD18 in 56 cases of gastric adenocarcinoma and adjacent cancer-free tissues were respectively detected by RT-PCR, Western blotting and immunohistochemistry to analyze the relationship between CARD18 and the clinical pathological characteristics. Results RT-PCR and Western blotting showed that the level of CARD18 mRNA and protein was down-regulated the most significantly after apoptin treatment. The expression of CARDI8 in gastric adeno-carcinoma tissues was significantly higher than that in adjacent cancer-free tissues ( P 〈 0.0.5 ), and it was proved that the CARD18 expression was related to the gastric cancer lymph node metastasis and TNM stage ( P 〈 0.05 ). Conclusion CARD18 may be both a promising marker for prognosis and a target protein for treatment of gastric cancer.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2013年第8期858-861,865,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(30500234)