摘要
A new star-shaped oligoelectrolyte (TEFCOONa) with triphenylamine as the core, acetylene as linkage and anionic fluorenes as arms was obtained and used for direct imaging in living PANC-1 cells. Because of the hydrophobic conjugated groups of the oli-goelectrolyte, TEFCOONa can form nanospheres with an average diameter of^75 nm in 10 mmol/L PBS. These nanospheres possess a relatively high absolute quantum yield (16.5% in PBS), low cytotoxicity and can penetrate into the nucleus through the cytoplasm, which is essential for living cellular imaging. Collectively, these results validate our rational design of conjugated oligoelectrolyte and even hyper branched polymers-copolyelectrolyte as effective nanovectors for bioimaging and other clinical applications.
A new star-shaped oligoelectrolyte （TEFCOONa） with triphenylamine as the core, acetylene as linkage and anionic fluorenes as arms was obtained and used for direct imaging in living PANC-1 cells. Because of the hydrophobic conjugated groups of the oli- goelectrolyte, TEFCOONa can form nanospheres with an average diameter of -75 nm in 10 mmol/L PBS. These nanospheres possess a relatively high absolute quantum yield （16.5% in PBS）, low cytotoxicity and can penetrate into the nucleus through the cytoplasm, which is essential for living cellular imaging. Collectively, these results validate our rational design of conjugated oligoelectrolyte and even hyper branched polymers-copolyelectrolyte as effective nanovectors for bioimaging and other clinical applications.
基金
supported by the National Basic Research Program of China (2009CB930600, 2012CB933301 and 2012CB723402)
the Ministry of Education of China (IRT1148)
the National Natural Science Foundation of China (21222404, 51173080 and 21104033)
the Program for New Century Excellent Talents in University (NCET-10-0179)
the Specialized Research Fund for the Doctoral Program of Higher Education(20093223110003)
the Natural Science Foundation of Jiangsu Province of China (BZ2010043 and NY211003)
the Program for Postgraduates Research Innovation of Colleges
Universities of Jiangsu Province(CXLX11_0424 and CXLX11_ 0424_0414)
