针刺内听宫穴对庆大霉素致聋大鼠听性脑干反应的影响

Effect of acupuncture treatment at Neitinggong acupoint on auditory brain-stem response in rats with gentamycin-induced hearing impairment

目的观察针刺内听宫穴对庆大霉素致聋大鼠听力的改善作用。方法 20只成年大鼠平均分为4组,庆大霉素组每日上午腹腔注射庆大霉素100 mg/kg,连续14 d;内听宫组庆大霉素给药方法同庆大霉素组,自腹腔注射庆大霉素第1天开始,隔日下午进行针刺内听宫穴,共10次;听宫组庆大霉素给药方法同庆大霉素组,自腹腔注射庆大霉素第1天开始,隔日下午进行针刺听宫穴,共10次;对照组每日上午腹腔注射等剂量生理盐水,共10次。分析实验前、实验14 d及20 d各组大鼠听性脑干反应阈,评价各组大鼠听力情况。结果庆大霉素组的听性脑干反应阈明显高于内听宫组、听宫组及对照组,内听宫组与听宫组听性脑干反应阈比较无明显差异。结论针刺内听宫穴及听宫穴均可在一定程度上降低庆大霉素致聋大鼠听性脑干反应阈,减轻庆大霉素耳毒性,缓解药物对听力的损害作用,是治疗药物性耳聋的有效穴位。

Objective To observe the effect of acupuncture at Neitinggong acupoint on the gentamycin-induced deafness. Methods 20 rats were randomly divided into 4 groups of five. Gentamycin group received an intraperitoneal injection of gentamycin every morning in a dose of 100 mg/kg for 14 consecutive days. Neitinggong group and Tinggong group received intraperitoneal injection of gentamycin as the same as the Gentamycin group, and were given acupuncture at Neitinggong or Tinggong separately once every two days from the first day of injection for a total of 10 times. Control group received intraperitoneal injection of normal saline of the same volume of gentamycin every morning without other interventions. Auditory brainstem response （ABR） examination was performed in all animals at day one, the 14th day and the 20th day to analyze their ABR thresholds. Results The ABR threshold of Gentamycin group was significantly higher than those of the Neitinggong group, the Tinggong group and the control group separately. The difference of ABR threshold between the Neitinggong group and the Tinggong group was statistically insignificant. Conclusions Acupuncture at Neitinggong and Tinggong acupoints can decrease the auditory brainstem response threshold and therefore reduce gentamycin-induced ototoxicity to some extent, which indicates that the Neitinggong acupoint may be an effective acupoint for treatment of gentamycin-induced deafness.