宫颈鳞癌组织SLP-2表达临床意义初步研究

Expression of SLP-2 in the cervical squamous cell carcinoma and its significance

目的:研究SLP-2在宫颈鳞癌组织中的表达及其与临床病理参数之间的关系。方法:收集郑州大学第二附属医院经手术切除的组织42例宫颈鳞癌组织、43例宫颈上皮内瘤变(CIN)患者的宫颈组织和48例正常宫颈组织,采用半定量RT-PCR技术检测SLP-2mRNA的表达情况,以上组织在病理科存档的石蜡切片采用免疫组化SP法检测SLP-2蛋白的表达情况。结果:PT-PCR法检测结果显示,与CIN及正常宫颈组织相比,SLP-2mRNA在宫颈鳞癌组织中的表达水平显著降低,F=3 979.485,P<0.01;免疫组化染色结果显示,SLP-2蛋白在正常宫颈组织中的表达率81.3%,CIN组织中的表达率为51.2%,宫颈鳞癌组织中的表达率(14.3%)明显低于CIN及正常宫颈组织,χ2=40.197,P<0.01;SLP-2的低表达与宫颈鳞癌的组织分化程度相关(P=0.033),而与患者年龄、临床分期、淋巴结转移、肌层浸润深度及肿瘤大小无相关性,P值均>0.05。结论:SLP-2在宫颈鳞癌中低表达,SLP-2的低表达在宫颈鳞癌的发生发展中发挥重要的作用,可能与宫颈鳞癌的组织分化程度密切相关。

OBJECTIVE：To study the expression of atomatin-like protein-2（SLP-2） in the cervical squamous cell car cinoma（CSCC）,and analyze the correlation between SLP-2 expression and clinicopathotogical features. METHODS： The expression of SLP-2 in 42 case of CSCC and 43 case of cervical intraepithelial neoplasia （CIN） and 48 case of normal cervical epithelium at the second affilated hospital of zhengzhou university was examined by semi-quantitative RT-PCR and Streptavidin-Peroxidase（S-P） immunohistochemical technique. RESULTS： The expression of SLP-2mRNA in CSCC was significantly lower than that in CIN and normal cervical epithelium（F= 3 979. 485, P〈0.01） ;immunohistochemical re- vealed that the expression rate of SLP-2 protein in normal cervical epithelium was 81.3% and in CIN was 51.2% and in CSCC was 14.3 , the expression rate of SLP-2 protein in CSCC was significantly lower than that in CIN and normal cer vical epithelium（ X.2 = 40. 197, P（0.01 ） ;SLP-2 low-level expression correlates with the degree of CSCC histological differentiation （ P = 0. 033）, but no with other clinicopathological features（ P〈 0.05）. CONCLUSION ： SLP-2 may play an important role in tumorigenesis of CSCC,The lower expression of SLP-2 may be closely associated with the degree of CSCC histological differentiation.