核抗原Mina53在人胃癌细胞中的作用及其意义探讨

Effect and Significance of Nuclear Antigen Mina53 on Human Gastric Carcinoma Cells

背景:核抗原Mina53基因为原癌基因Myc的下游直接靶基因之一,在一些消化系统恶性肿瘤中呈高表达,并与肿瘤增殖、侵袭、转移或患者生存期相关。目的:研究Mina53在人胃癌细胞中的作用及其对胃癌发生、发展的意义。方法:选择Mina53表达水平较高的人胃癌细胞株SGC7901和AGS,应用RNA干扰技术下调其Mina53表达,以转染无关序列siRNA的细胞作为对照组。采用CCK-8实验检测细胞增殖,流式细胞术检测细胞周期和细胞凋亡,细胞侵袭和迁移实验检测细胞侵袭、迁移能力。结果:与相应对照组相比,Mina53 siRNA转染组SGC7901、AGS细胞增殖受抑(96 h相对增殖率:60%和68%),并发生明显细胞周期G1期阻滞(SGC7901细胞G1/G2:2.76±0.12对1.86±0.06,P<0.05;AGS细胞G1/G2:1.78±0.13对1.34±0.05,P<0.05),细胞凋亡率分别增加9.8%±1.2%和10.6%±1.5%(P<0.05),穿透Transwell小室基质胶细胞数(SGC7901细胞:11.67±0.88对24.33±1.45,P<0.05;AGS细胞:8.00±1.15对20.33±1.73,P<0.05)和穿透Transwell小室微孔膜细胞数(SGC7901细胞:7.00±1.53对14.67±2.03,P<0.05;AGS细胞:8.00±1.16对15.33±1.45,P<0.05)均显著减少。结论:Mina53对人胃癌细胞的增殖、细胞周期、细胞凋亡以及侵袭、迁移能力具有调控作用,可能影响胃癌的生长、浸润和转移,有望作为胃癌基因治疗的靶点。

Background： As a downstream direct target gene of oncogene Myc,nuclear antigen Mina53 is over-expressed in some carcinomas of digestive system,and is demonstrated to be related with tumor proliferation,invasiveness,metastasis and survival of patients.Aims： To investigate the effect of Mina53 on human gastric carcinoma cells and its significance in the occurrence and development of gastric carcinoma.Methods： SGC7901 and AGS,two human gastric carcinoma cell lines with high expression levels of Mina53,were selected and transfected with Mina53 siRNA to knock down Mina53.Cells transfected with siRNA containing inert sequence were served as controls.Cell proliferation was assessed by CCK-8 assay,cell cycle and apoptosis were analyzed by flow cytometry,and capacities of cell invasion and migration were detected by cell invasion and migration assays.Results： Transfection with Mina53 siRNA significantly repress the proliferation of SGC7901 and AGS cells with a relative proliferation rate of 60% and 68%（96 h）,respectively;knocking down Mina53 also arrested the cell cycle at G1 phase（G1 / G2 ratio in SGC7901 cells： 2.76 ± 0.12 vs.1.86 ± 0.06,P〈0.05;in AGS cells： 1.78 ± 0.13 vs.1.34 ± 0.05,P〈0.05） and increased the apoptosis rates of SGC7901 and AGS cells by 9.8% ± 1.2% and 10.6% ± 1.5%,respectively（P〈0.05）.Meanwhile,knocking down Mina53 significantly inhibited the invasion and migration of SGC7901（invasion cells： 11.67 ± 0.88 vs.24.33 ± 1.45,P〈0.05;migration cells： 7.00 ± 1.53 vs.14.67 ± 2.03,P〈0.05） and AGS cells（invasion cells： 8.00 ± 1.15 vs.20.33 ± 1.73,P〈0.05;migration cells： 8.00 ± 1.16 vs.15.33 ± 1.45,P〈0.05） in Transwell chamber.Conclusions： Mina53 can regulate proliferation,cell cycle,cell apoptosis,and invasion and migration capacities of human gastric carcinoma cells,which indicates that it plays an important role in the growth,invasiveness and metastasis of gastric carcinoma and may be considered as a target of gastric carcinoma gene therapy.