氨溴索对铜绿假单胞菌密度感应缺陷株生物膜细菌活力和黏附作用的影响

Effects of ambroxol on biofilm adhesion and viability of Pseudomonas aeruginosa quorum sensing defective strain

目的研究氨溴索对铜绿假单胞菌野生株PAO1及密度感应缺陷株(lasI rhlI基因缺陷)生物膜(BF)细菌活力及早期黏附的影响。方法利用平板计数法测定不同浓度(0、1.875、3.75mg/ml)氨溴索对PAO1菌株、lasI rhlI菌株生物膜菌落的清除效应;荧光多功能酶标仪检测不同浓度氨溴索作用后PAO1菌株、lasI rhlI菌株的荧光强度,计算黏附率以反映干预对细菌黏附的影响。结果氨溴索作用后,两种菌株的活菌生存率和黏附率均出现下降,lasI rhlI株活菌生存率较PAO1株下降更明显(P<0.05),而PAO1株黏附率较lasI rhlI株下降更明显(P<0.05)。结论氨溴索具有拮抗密度感应系统的作用,可用于抗细菌生物膜活力,为难治性铜绿假单胞菌感染的治疗提供了新思路。

Objective To investigate the e ects of ambroxol on the bio lm viability and pristine adhesion of Pseudomonas aeruginosa wild（PAO1） and quorum sensing defective strain（QS,gene deletion of lasI and rhlI）.Methods e bio lm was treated by different concentrations（0,1.875,3.75mg/ml） of ambroxol.The number of colony was measured with agar plate,multifunction uorometer was used to measure the uorescence intensity of PAO1 and QS strains at the bo om of 96-well plate.e adhesion ratio（%） was calculated to determine the e ects of ambroxol on bacterial bio lm adhesion.Results Ambroxol treatment reduced the survival rate of the mutant strains compared to that of wild strain,even though the QS strain had increased the adhesion in the presence of ambroxol compared to that of wild strain（P＆lt;0.05）.Conclusion Ambroxol has a property of signi cantly antagonizing quorum-sensing system,suggesting that it might be of importance in treatment against chronic Pseudomonas aeruginosa infections.