血必净对兔创伤性脑损伤脑细胞凋亡的影响

Effects of Xuebijing on apoptosis induced by traumatic brain injury in rabbits

目的评价血必净对兔创伤性脑损伤脑细胞凋亡的影响。方法雄性健康新西兰大耳白兔36只，4—5个月龄，体质量2．1～2．5kg，采用随机（随机数字法）数字表法将其随机分为三组（n=12）：假手术组（S组）、损伤组（T组）和血必净治疗组（x组）。T、X组采用改良Feeney法建立创伤性脑损伤模型，S组只钻出骨窗不打击脑组织。T、X组在制模成功后24h采集兔损伤部位脑组织标本，S组于相同时间点采集兔对应部位脑组织标本。采用TUNEL法检测脑细胞凋亡情况，Western blot法检测脑组织Bcl-2、Bax蛋白表达。结果与S组比较，T、x组脑细胞凋亡率升高，Bcl-2／Bax蛋白表达比值降低，差异具有统计学意义（P〈0．01）；与T组比较，x组脑细胞凋亡率降低，Bcl-2／Bax蛋白表达比值升高，差异具有统计学意义（P〈0．01）。结论血必净可一定程度降低兔创伤性脑损伤脑细胞的凋亡，从而发挥脑保护作用，其机制可能与调节Bcl-2／Bax蛋白功能向抗凋亡方向转移有关。

Objective To evaluate the effects of Xuebijing on apoptosis induced by traumatic brain injury in rabbits. Methods A total of 36 healthy adult male rabbits were randomly devided into 3 groups （n = 12） ： sham group （group S）, traumatic brain injury group （group T）, and Xuebijing therapy group （group X）. The model of traumatic brain injury was produced by improved Feeney＇ s traumatic brain injury model. Group S drill bone window and not crack down on brain tissue. The rabbits were sacrificed for injury brain tissue samples at 24 h after molding success in every groups. Brain cell apoptosis was evaluated by TUNEL staining. The cerebral expression of Bcl-2 and Bax was analyzed by Western blot. Results Compared with group S, the rates of apoptosis were increased and the protein expression ratios of Bcl-2/Bax were decreased in group T, and group X （P 〈0. 01 ）. Compared with group T, the rates of apoptosis were decreased and the protein expression ratios of Bcl-2/Bax were increased in group X （P 〈 0. 01 ）. Conclusions Xuebijing can reduce the apoptosis of cerebral cells induced by traumatic brain injury to a certain extent, and protect the cerebrum, the signal of Bcl-2/Bax change to the way of anti-apoptosis is involved in the mechanism.