摘要
目的评价血必净对兔创伤性脑损伤脑细胞凋亡的影响。方法雄性健康新西兰大耳白兔36只,4—5个月龄,体质量2.1~2.5kg,采用随机(随机数字法)数字表法将其随机分为三组(n=12):假手术组(S组)、损伤组(T组)和血必净治疗组(x组)。T、X组采用改良Feeney法建立创伤性脑损伤模型,S组只钻出骨窗不打击脑组织。T、X组在制模成功后24h采集兔损伤部位脑组织标本,S组于相同时间点采集兔对应部位脑组织标本。采用TUNEL法检测脑细胞凋亡情况,Western blot法检测脑组织Bcl-2、Bax蛋白表达。结果与S组比较,T、x组脑细胞凋亡率升高,Bcl-2/Bax蛋白表达比值降低,差异具有统计学意义(P〈0.01);与T组比较,x组脑细胞凋亡率降低,Bcl-2/Bax蛋白表达比值升高,差异具有统计学意义(P〈0.01)。结论血必净可一定程度降低兔创伤性脑损伤脑细胞的凋亡,从而发挥脑保护作用,其机制可能与调节Bcl-2/Bax蛋白功能向抗凋亡方向转移有关。
Objective To evaluate the effects of Xuebijing on apoptosis induced by traumatic brain injury in rabbits. Methods A total of 36 healthy adult male rabbits were randomly devided into 3 groups (n = 12) : sham group (group S), traumatic brain injury group (group T), and Xuebijing therapy group (group X). The model of traumatic brain injury was produced by improved Feeney' s traumatic brain injury model. Group S drill bone window and not crack down on brain tissue. The rabbits were sacrificed for injury brain tissue samples at 24 h after molding success in every groups. Brain cell apoptosis was evaluated by TUNEL staining. The cerebral expression of Bcl-2 and Bax was analyzed by Western blot. Results Compared with group S, the rates of apoptosis were increased and the protein expression ratios of Bcl-2/Bax were decreased in group T, and group X (P 〈0. 01 ). Compared with group T, the rates of apoptosis were decreased and the protein expression ratios of Bcl-2/Bax were increased in group X (P 〈 0. 01 ). Conclusions Xuebijing can reduce the apoptosis of cerebral cells induced by traumatic brain injury to a certain extent, and protect the cerebrum, the signal of Bcl-2/Bax change to the way of anti-apoptosis is involved in the mechanism.
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2013年第7期741-743,共3页
Chinese Journal of Emergency Medicine
基金
浙江省中医药科技计划项目(2009CB043)