摘要
为了解吉林省牛瑟氏泰勒虫病的流行情况,本研究采用PCR方法和血涂片染色镜检法对采自吉林省6个地区的234份牛血液样品进行了检测,并对不同地区、不同饲养方式、不同地理条件以及不同年龄间牛瑟氏泰勒虫的感染情况进行了比较分析。结果显示,PCR方法检测的样本阳性率为53.4%(125/234),而血涂片染色镜检法检测的阳性率为12.4%(29/234)。经统计学分析,不同年龄间,牛瑟氏泰勒虫感染率差异显著(p<0.05);而不同地区、不同饲养方式以及不同地理条件之间牛瑟氏泰勒虫感染率差异极显著(p<0.01);调查结果表明,吉林省是牛瑟氏泰勒虫病的流行地区,尤其珲春地区牛瑟氏泰勒虫病感染严重。本次调查为吉林省牛瑟氏泰勒虫病的防控提供了依据。
In order to understand the prevalence of Theileria sergenti infections in cat-tile in Jilin province, molecular epidemiological survey was conducted using PCR and microscopic examination method. Risk factors such as regions, grazing, geographical conditions and ages were evaluated and the data was statistically analyzed. The results showed that the T.sergenti positive rates were 53.4% (125/234), and detected by PCR and microscopic examination 12.4% (29/234), respectively. Statistical analysis showed that the positive rate was significantly different in different ages (p〈0.05), while the positive rate was significant difference in different regions, feeding model and geographical conditions (p〈0.01). The results of this survey indicated that Jilin province, especially Hunchun area, was the endemic area.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2013年第7期539-541,共3页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然科学基金(30960278)
吉林省自然科学基金(201115230)