人胚胎早期大脑额叶脑源性神经营养因子阳性神经元的表达

Morphological Study on Early Development of Brain Derived Neurophic Factor-positive Neurons in the Frontal Lobe of Human Fetus

目的探讨人胚胎早期大脑额叶脑源性神经营养因子(BDNF)阳性神经元的表达规律。方法采用免疫组织化学SABC方法分别检测2、3、4个月龄段BDNF在人胚胎大脑额叶组织中的表达。结果第2个月龄时,大脑额叶室管层可见到BDNF阳性神经元分布;第3个月龄时,中间层BDNF阳性神经元形态各异,核大,胞质少,有突起;第4个月龄时,中间层BDNF阳性神经元胞体增大,胞质相对增多,染色加深,神经元之间有神经纤维分布,膨体颗粒明显,大脑额叶边缘层可见BDNF阳性神经元。结论 BDNF阳性神经元参与了人胚胎早期大脑额叶的发育。

Objective To investigate the growth and development of brain derived neurophic factor （BDNF） -positive neurons in the frontal lobe of human fetus. Methods The expression of the BDNF-positive neurons in the frontal lobe of human fetus in the 2^na, 3^ra, and 4^th month of gestation were observed with the streptavidin-biotin-eomplex/immunoperoxidase （ SABC ） method. Results By the second month of gestation, BDNF-positive neurons were seen in the subventricular layer of the frontal lobe of cerebellum. By the third month of gestation, BDNF-positive neurons in the central layer were in various shapes, with big nucleus, less cyto- plasm, and small processes. By the fourth month of gestation, BDNF-positive neurons in the central layer grew larger in size, cytoplasm increased, the BDNF-positive expression was enhanced with deeper dyeing, and the nerve fibers and particles were distributed between neurons; also, the BDNF-positive neurons were seen in the marginal layer of the frontal lobe of cerebrum. Conclusion BDNF-positive neurons may participate in the early development of the frontal lobe of cerebrum of human fetus.