上皮型钙黏附蛋白和Snail在原发性干燥综合征患者唇腺组织中表达的相关研究

The study of the expression of E-cadherin and Snail in the labial glands of the patients with primary Sjiigren＇s syndrome

目的探讨锌指转录因子Snail、上皮型钙黏附蛋白（E-cad）在原发性干燥综合征（pSS）发病过程中的作用和意义。方法采用免疫组织化学链霉素抗生物素．过氧化酶法（SP法）检测50例pSS患者及30名健康对照组唇腺组织中Snail、E-cad的表达情况。2组间阳性率的比较采用χ^2检验或Fisher’s确切概率法，相关性分析采用Spearman相关分析法。结果E-cad在正常唇腺组织中阳性表达率为100％（30／30），而在pss患者唇腺组织中阳性率为62％（31／50），2组间差异具有统计学意义（χ^2=14．651，P〈O．01）；Snail在正常唇腺组织阳性表达率为0，而50例pSS患者唇腺组织中20例呈阳性表达（阳性表达率为40％），2组间差异有统计学意义（χ^2=28．800，P〈0．01）；在pSS组患者唇腺组织中随着淋巴细胞浸润等级的增加，E-cad表达降低（P〈O．01），而Snail的表达增强（P〈0．01）；pSS组患者唇腺组织中E-cad与Snail表达呈负相关（rs=-0．484，P〈O．01）。结论Snail和E-cad与pSS患者唇腺组织的破坏有关，可能参与了pSS的发病过程。

Objective To study the expression of E-cadherin protein and Snail protein in the labial glands of patients with primary Sjogren＇s syndrome（pSS） and to analyze their roles in the pathogenesis of pSS. Methods The expression of E-cadherin protein and Snail protein were detected by immunohistochemical method. The labial glands were obtained from 50 patients with pSS and 30 healthy controls. The data was analyzed by Chi-Square test, Fisher＇s exact probability and Spearman＇s rank correlation analysis. Results The expression rate of E-cadherin in the labials gland of the patients with pSS were lower than that in normal salivary tissues （χ^2=14.651, P〈0.01 ）. The positive rates of Snail in the labial glands of the patients with PSS were 40% and it＇s expression rates were significantly higher than those in normal salivary tissues （χ^2=28.800, P〈0.01）. There was a negative correlation between lymphocyte focus score and the expression of E-cadherin （P〈0.01） and a positive correlation between lymphocyte focus score and the expression of Snail in pSS group （P〈0.01）. There was a negative correlation between the expression of E-cadherin and Snail （rs=-0.484, P〈 0.01）. Conclusion The interaction of E-cadherin and Snail may have played an important role in the Dathozenesis of PSS.