MAPK在病理性瘢痕和瘢痕癌中的表达

The Correlation Research about MAPK with the Pathological Scar and Scar Cancer

目的探讨MAPK及其mRNA与病理性瘢痕上皮和瘢痕癌的相关性。方法采用免疫组织化学(SP)法和核酸分子原位杂交技术分别检测正常皮肤表皮,病理性瘢痕上皮和瘢痕癌三组组织中MAPK蛋白和mRNA的表达;结合图像分析,分别计算三组组织中所检指标的平均光密度和阳性面积,所有数据运用SPSS16.0软件包进行统计学分析。结果 MAPK蛋白和mRNA在正常皮肤表皮、病理性瘢痕上皮和瘢痕癌组织中分别呈阴性表达、弱阳性表达和强阳性表达。瘢痕癌组表达水平(阳性面积)、表达强度(平均光密度)与正常皮肤、病理性瘢痕组比较,差异均有统计学意义(P均<0.01);但正常皮肤组与病理性瘢痕组比较,差异无统计学意义(P>0.05)。且在瘢痕癌组织中,MAPK及其mRNA的表达呈正相关。结论 MAPK及其mRNA在瘢痕癌中的高表达,与瘢痕癌的发生有相关性,可作为瘢痕癌靶向治疗的靶点,但在病理性瘢痕上皮中的表达不具有特殊意义。

Objective Explore the correlation about the MAPK and MAPK mRNA with the pathological scar cancer and scar carcinoma. Methods The MAPK protein and MAPK mRNA were detected in normal skin epidermis, pathological scar epithelium and scar cancer three groups tissues by the immunohistochemical method of S-P and in situ hybridization. The analysis of image were combinted, and calculated the optical density and positive area indicators seized in the inspection organization, and all data was input into the computer and statistically analyzed with SPSS16.0 software. Results MAPK and its mRNA showed negative expression in nor- mal skin epidermis, showed weakly positive expression in pathological scar epithelium, showed strongly posi- tive expression in scar carcinoma tissue. The expression of levels （positive area） and intensity （mean optical densit） differences were statistically significant （ P 〈 0.01 ） by compared with normal skin group and skin scar group. But the differences of expression has no statistically significant between normal skin group and pathological scar group. Correlated analysis showed： it was positive correlation between the expression levels of MAPK and its mRNA. Conclusion The MAPK protein and its mRNA showed strong positive expression in the scar cancer tissue, which may be play important roles in the occurrence of scar carcinoma, can be used as scar cancer targeted therapy target. The expression of MAPK protein and its mRNA in pathological scar epithelium does not have a special meaning.