健康成年男性泌尿生殖道解脲脲原体及人型支原体的定植状况调查

Detection of Ureaplasma Urealytium and Mycoplasma Homanis in Urogenital Tract in Healthy Men

目的了解解脲脲原体(UU)及人型支原体(Mh)在健康成年男性泌尿生殖道中的定植情况,并揭示UU各生物群/血清型在该人群中的分布特征。方法将尿沉渣接种于液体培养基中进行培养,对培养阳性者进行PCR反应,并进一步对UU阳性者进行分群分型。结果 800份标本按年龄分三组:青年组21～44岁,平均(38.91±2.45)岁;中年组45～59岁,平均(46.21±5.62)岁;老年组≥60岁,平均(69.85±7.44)岁。共检出UU95例(11.88%):青年组31例(12.40%),中年组48例(19.20%),老年组16例(5.33%)。青年组、中年组UU检出率显著高于老年组,差异均有统计学意义(P<0.05)。三组UU均以单纯生物一群定植为主。青年组和中年组以6型为主,老年组以3/14型为主。Mh共检出14例(1.75%):青年组6例(2.40%),中年组7例(2.80%),老年组1例(0.33%)。结论 UU尤其是生物一群可能是健康成年男性泌尿生殖道中的正常定植菌群,6型和3/14型为青岛地区健康成年男性优势血清型。Mh在健康成年男性中携带率较低。

Objective To investigate the detection rate of Ureaplasma urealyticum （UU） and Mycoplasma hominis（Mh） in urogenital tract of healthy men and the biovars and serotypes of UU. Methods The forepart urine samples were collected from 800 healthy men who visited Qingdao Municipal hospital for physical check-up between April and June;all samples were cultured by UU and Mh liquid mediums separately. The UU and Mh positive cases were then amplified by Polymerase Chain Reaction （PCR）. The biovars and serotypes of UU were detected by PCR. Results Among the youth,31 cases （12.40%） were UU positive： 20 cases were Parvum biovar and 12 were urealytieum biovars;6 cases （2.40%） were Mh positive by PCR. Among the middle aged men, 48 cases （19.20%） were UU positive： 30 cases were Parvum biovar and 19 cases were urealytieum biovars ;7 cases （2.80%） were Mh positive by PCR. Among the old men, 16 cases （5.33%） were UU positive： 10 cases were Parvum biovar and 6 cases were urealytieum biovars, 1 cases （0.33%） were Mh positive by PCR. Conclusion UU are commensal microorganism in urogenital tract in healthy men. The Parvum biovar is predominant biovar in all the three groups. It presumes that UU, especially the parvum biovar is possibly carried by healthy men. The seretype 6 and serotype 3/14 are predominant serotypes of healthy women. The detection of Mh is very low inhealthy women.