河北卢龙地区猪C组轮状病毒流行状况和VP6基因多样性分析

The epidemiological characteristics of group C rotavirus in Lulong area and the analysis of diversity of VP6 gene

目的 了解GCRV在我国卢龙地区猪中流行状况和VP6蛋白编码基因的多样性特点,为猪GCRV的深入研究提供依据.方法 采集2007年冬至2008年春河北卢龙地区腹泻和正常猪粪便标本共793份.采用巢式反转录-聚合酶链反应（Nested RT-PCR）的方法对GCRV进行检测,并对测定序列进行同源性和系统进化分析.结果 在793份粪便标本中,GCRV阳性率为16.65％,卢龙地区猪GCRV毒株间同源性差异较大.核苷酸进化树表明,猪GCRV多样性显著.由氨基酸进化分析可知,同一宿主的GCRV氨基酸序列亲缘关系更近.结论 2007-2008年冬春两季,C组轮状病毒在我国卢龙地区猪中感染率较高.VP6基因多样性广泛存在.实验提供的数据为进一步研究猪GCRV的分子流行特征提供了基础.

Objective To study the epdimiology characteristics and the diversity of VP6 gene of GCRV in Lulong,and to provide the basis for GCRV in-depth research.Methods 793 stool specimens from porcine with diarrhea or not from Lulong in 2007 and 2008.GCRV was detected by nested multiple reverse transcription-polymerase chain reaction （nested RT-PCR）,and analyzed the identity and conducted phylogenetic tree by the seqences.Results The positive rate of GCRV was 16.65％.Porcine GCRV strains of Lulong had significant homology differences.Phylogenetic analysis indicated porcine GCRVs were with significant diversity.Amino acid analysis showed GCRV strains with the same host shared the nearest kinship.Conclusion The infection rate of GCRV was high from 2007 to 2008 in Lulong.Homology and phylogenetic analysis showed that VP6 gene diversity was widespread.The experimental data provided basis for molecular characteristics of porcine GCRVs.