MicroRNA-126对卵巢癌细胞SKOV3迁移和侵袭能力的影响

Effect of MicroRNA-126 on migration and invasion of ovarian cancer cell line SKOV3

目的:探讨microRNA-126(miR-126)对卵巢癌细胞株SKOV3迁移和侵袭能力的影响,及其对人脐静脉内皮细胞(HUVECs)体外血管形成能力的影响。方法:利用脂质体瞬时转染miR-126 mimics、mimics NC于SKOV3细胞。Real-time PCR法检测卵巢癌组织及转染前后SKOV3细胞中miR-126的表达,Tanswell小室检测SKOV3细胞的迁移和侵袭情况。分离培养原代HUVECs,利用三维小管形成实验检测HUVECs体外血管形成情况。结果:卵巢癌组织中miR-126的相对表达量(0.29±0.38)显著低于正常卵巢上皮组织(1.18±0.47)(P<0.01)。miR-126 mimics组SKOV3细胞的miR-126相对表达量(5.15±1.00)显著高于未处理组(1.07±0.27)、NC组(0.99±0.20)。转染后,SKOV3细胞的迁移和侵袭能力均显著降低,但HUVECs三维成管数无显著变化。结论:miR-126表达上调能抑制卵巢癌细胞SKOV3的体外迁移和侵袭能力,miR-126低表达可能与卵巢癌的疾病进展有关。

Objective：To investigate the effects of microRNA-126 on the migration and invasion of human ovarian cancer cell line SKOV3 and the angiogenic ability of human umbilical vein endothelial cells（HUVECs） in vitro.Methods：Quantitative real-time PCR was used to analyze the expression of miR-126 in epithelial ovarian cancers and SKOV3 cells which were transfected with miR-126 mimics or miR-control（NC） by Lipofectamine 2000.Transwell chamber assay was used to detect the migration and invasion ability of cells.Isolating and culturing HUVECs in vitro.Angiogenic ability of HUVECs in vitro was detected by in vitro tube formation assay on matrigel.Results：The expression of miR-126 in epithelial ovarian cancer tissues（0.29±0.38）was significantly lower than that in normal ovarian tissues（1.18±0.47）.Up-regulation of miR-126 could reduce the number of cells which migrated and invaded through the membrane of the transwell chamber.But the numbers of tubular structures formed by HUVECs had no significant differences.Conclusion：Up-regulation of miR-126 in human ovarian cancer cell can inhibit the migration and invasion of tumor cells in vitro,suggesting that the down-regulation of miR-126 in the ovarian cancer cells contributes to the progression of disease.