ALX-R在子痫前期患者胎盘组织中的表达及其与NF-κB p65的相关性

Expression of the lipoxin A4 receptor in placenta with preeclampsia and its correlation with nuclear factor-κb p65

目的:观察子痫前期(PE)患者胎盘组织中脂氧素A4受体(ALX-R)、核因子-κB p65(NF-κB p65)表达的变化,探讨两者在PE发生发展中的作用及相关性。方法:采用RT-PCR方法、免疫组化法检测PE组(轻度10例、重度20例)与对照组(20例)孕妇胎盘组织中ALX-R、NF-κB p65 mRNA和蛋白的表达水平。结果:(1)胎盘组织中ALX-RmRNA表达水平随病情严重程度而明显下降,重度PE组低于轻度PE组、对照组(P<0.01);NF-κB p65 mRNA表达水平随病情严重程度而升高,重度PE组高于轻度PE组、对照组(P<0.05);(2)胎盘组织中PE组ALX-R蛋白的表达强度低于对照组(P<0.01);PE组NF-κB p65蛋白的表达强度高于对照组(P<0.01);(3)重度与轻度PE组胎盘组织中ALX-R与NF-κB p65的mRNA和蛋白表达水平无明显相关性;对照组胎盘组织中ALX-R与NF-κB p65的mRNA和蛋白表达水平呈负相关(r=-0.86,P<0.01;r=-0.63,P<0.01)。结论:ALX-R与NF-κB p65的表达,可能与PE发生发展的病理生理过程密切相关。

Objective：To investigate the expression and its significance of lipoxin A4 receptor and the transcription factor NF-κB p65 in placenta of patients with preeclampsia.Methods：PCR and immunohistochemistry were used to detect the expression of mRNA and protein for ALX-R and NF-ΚB p65 in the placenta of 30 women with preeclampsia（including 10 cases of mild preeclampsia and 20 cases of severe preeclampsia） and 20 normal full-term pregnant women（control group）,respectively.Results：（1）The expression of ALX-R mRNA in placenta was decreased significantly with disease severity,it was lower in severe preeclampsia placenta than in mild preeclampsia and control group（P〈0.01） The expression of NF-ΚB p65 mRNA in placenta was increased significantly with disease severity,it was higher in severe preeclampsia placenta than in mild preeclampsia and control group（P〈0.05）.（2）The expression of ALX-R protein showed significant lower in placenta with preeclampsia than in control group（P〈0.01）,and the expression of NF-κB p65 protein showed significant higher in placenta with preeclampsia than in control group（P〈0.01）.（3）There was no significant correlated between the expression of ALX-R and NF-κB p65 mRNA and protein in placenta with severe preeclampsia and mild preeclampsia.There was negative correlated between with the expression of ALX-R and NF-κB p65 mRNA and protein in placenta with control group（P〈0.05）.Conclusion：The abnormal expressions of ALX-R and NF-κB p65 in placenta may play an important role in the pathogenesis of preeclampsia.