以1,4-二甲基咔唑-9-丙酸为荧光衍生化试剂高效液相色谱法测定醇

Determination of alcohols by HPLC with 1,4-dimethylcarbazole-9-yl-propionic acid as fluorescence derivative reagent

1,4-二甲基咔唑-9-丙酸与间苯二磺酰氯反应生成混合酸酐,该混合酸酐能够衍生化18个碳以下的直链脂肪伯醇、6个碳以下的仲醇,以及叔丁醇和胆固醇;将荧光衍生物用反相C18液相色谱柱进行分离,在室温下10min完成衍生化反应,衍生物24h内基本稳定.基于此,建立了血清中醇的高效液相色谱测定方法.

Pre-column derivative reagent 1,4-dimethyl-carbazole-9-yl-propionic acid was allowed to couple with benzenedisulfonyl dichloride affording the mixed acidic anhydride. Resultant mixed acidic anhydride was used to derivate straight-chain aliphatic primary alcohols with less than 18 carbons and secondary alcohols with less than 6 carbons, as well as tert-butanol and cholesterol. As-obtained fluorescence derivatives were separated on reversed-phase C18 high- performance liquid chromatography （HPLC） column, with which the derivatization was fin- ished in 10 min at room temperature and relevant derivatives were stable within 24 h. This makes it feasible to establish a HPLC method to determine alcohols in human serum with 1,4- dimethyl-carbazole-9-yl-propionic acid as the fluorescence derivative reagent.