不同激素对母犬的诱导发情和产仔效果的研究

Effects of Different Hormones on Estrus Induction and Farrowing Effect of Bitches

为了研究不同激素对诱导母犬发情和产仔效果的影响,选用110只长期不发情的母犬作为试验动物,试验共分5个组,试验Ⅰ组肌肉注射PMSG,试验Ⅱ组使用PMSG和HCG组合分别肌肉注射,试验Ⅲ组使用PMSG和LRH-3组合分别肌肉注射,试验Ⅳ组使用PMSG、PGF2α、E2和LRH-34种药物组合分别肌肉注射,另设对照组,分别观察诱导发情和产仔效果。结果表明,在发情表现方面,试验Ⅳ组效果最好,在阴门肿胀程度、流血性分泌物及阴道上皮角化程度等方面均表现为高度反应;在发情率方面,试验Ⅳ组分别比试验Ⅰ组、试验Ⅱ组和试验Ⅲ组提高了32%、28%和20%,差异极显著(P<0.01);在受胎率方面,试验Ⅳ组的受胎率最高,为76%,分别比试验Ⅰ组、试验Ⅱ组和试验Ⅲ组提高了35%、20.4%和10%,试验Ⅰ组、试验Ⅱ组和试验Ⅳ组之间差异极显著(P<0.01),试验Ⅲ组和试验Ⅳ组之间差异极显著(P<0.01);在产仔数方面,试验Ⅳ组共产仔130只,分别比试验Ⅰ组、试验Ⅱ组、试验Ⅲ组多产92、74和45只,差异极显著(P<0.01)。对照组没有使用任何药物,发情率最低,只有10%,受胎率为0。试验结果显示,PMSG、PGF2α、E2、LRH-34种激素药物联合使用促进发情效果最好,在受胎率、产仔数方面均明显优于单独或其他组合使用的效果。

In order to study effects of different hormones on estrus induction and farrowing effect of bitches, 110 bitches without estrus for a along time were divided into five groups, among them group Ⅰ was treated with intramuscular injection of PMSG; group Ⅱ was treated with intramuscular injection of the combination of PMSG and HCG; group Ⅲ was treated with intramuscular injection of the combination of PMSG and LRH-3; group IV was treated with intramuscular injection of the combination of PMSG, PGF2α, E2 and LRH-3; group V was control group. Results showed that, as for estrus, group IV had the best effect, and showed high reaction in degree of vulva swelling, flowing-blood secretion and keratinization degree of vaginal epithelium; the estrus rate of group Ⅳ was 32%, 28% and 20% more than that of group Ⅰ, group Ⅱ and group Ⅲ respectively （P〈0.01）. Group IV had the highest conception rate of 76% which was 35%, 20.4% and 10% more than that of group Ⅰ, group Ⅱ and group Ⅱ respectively; group Ⅳ had very significantly difference with group Ⅰ and group Ⅰ, and had significantly difference with group Ⅲ. The litter size of group IV was 130 which was 92, 74 and 45 more than that of group Ⅰ , group Ⅱ and group Ⅲ, respectively （P〈0.01）. Control group wasn＇t treated with any drug, and it had the lowest estrus rate （10%）, and its conception rate was 0. It is suggested that the combination of PMSG, PGF2ct, E2 and LRH-3 had the best effect of estrus induction, and both conception rate and litter size were better than those of single use or the other combinations.