基于ITS2序列诊断临床深部真菌感染的初步研究

Identification of invasive fungal infections by sequence analysis of the internal transcribed spacer region 2

目的建立以ITS2为靶序列的PCR方法诊断临床深部真菌感染,并进行初步研究。方法用通用引物扩增纯菌落和临床标本的ITS2区域,对PCR产物测序,测序结果在GenBank中进行Blastn等分析,以此来鉴定真菌属种。结果 10种常见致病性真菌纯菌落和10例临床标本的DNA测序结果均与表型鉴定符合。结论以ITS2为靶序列的PCR可成为临床快速诊断深部真菌感染的重要方法之一。

Objective To investigative the feasibility of using sequencing of the ribosomal internal transcribed spacer 2 （ITS2） for identification of invasive fungal infections.Methods Amplification and sequencing of internal transcribed spacer 2 （ITS2） region of fungi DNA from fungal isolates and clinical specimens.Searches of current nucleotide databases in GenBank were carried out with the BLAST algorithm.Results 10 common fungal isolates were accurately detected and 83% （10/12） invasive fungal infections cases were identified.Conclusion PCR targeting internal transcribed spacer 2 permits rapid and reliable identification of fungal species from clinical specimens.