UF-1000i尿分析仪有形成分检测筛查尿路感染的临床价值

The clinical value in the detection of the visible components by the Sysmex UF-1000i urine flow cytometer for screening urinary tract infection

目的评估UF-1000i尿分析仪定量检测尿液有形成分在筛查尿路感染(UTI)中的临床价值。方法运用UF-1000i尿分析仪及AX-4030尿干化学分析仪对335份临床尿样本进行检测,同时作定量细菌培养。以临床诊断尿路感染的标准作为"金标准",评估不同检测方法的诊断效能,Kappa一致性检验评价不同检测方法的一致性。结果定量细菌培养的灵敏度和特异度最高,其次是UF-1000i白细胞计数。与"金标准"比较,细菌培养法具有极强的一致性,UF-1000i白细胞计数具有高度的一致性,AX-4030白细胞酯酶检测和UF-1000i细菌计数具有中度一致性,AX-4030亚硝酸盐检测仅具有微弱一致性。结论 UF-1000i尿分析仪有形成分计数适用于临床UTI的诊断。UF-1000i白细胞计数不仅具有高度的一致性,而且具有较高的灵敏度和特异度,操作简单且易于标准化,适用于门诊UTI的筛选。

Objective To evaluate the clinical values in the detection of the visible components by Sysmex UF-1000i Urine Flow Cytometer for Screening Urinary Tract Infection（UTI）.Methods 335 urine specimens were detected by UF-1000i urine analyzer,AX-4030 urine dry chemistry analyzer and urine culture respectively.All these samples are diganozed by the standard of clinical diagnosis of urinary tract infection.The diagnostic tests of UF-1000i,AX-4030 and urine culture were evaluated.Kappa test was applied to determine the concordance between these methods.Results Quantitative bacterial cultures had the highest sensitivity and specificity,followed by UF-1000i white blood cell（WBC）count.Compared with gold standard,urine culture had a very strong consistency.AX-4030 leukocyte esterase detection and UF-1000i bacteria count with moderate consistency,AX-4030 nitrite detection had only a weak consistency.Conclusion The UF-1000i detection of the visible components was suitable for clinical diagnosis in UTI due to its high sensitivity,specificity and simple operation,which was easy to be standardized and suitable for outpatients.