2635例腹泻患儿粪便中A群轮状病毒抗原检测结果分析

Analysis on detection results of group A rotavirus antigen in stool of 2 635 infants with diarrhea

目的对2635例婴幼儿腹泻患儿进行粪便A群轮状病毒抗原检测,为临床提供快捷、准确、可靠的诊断依据。方法应用免疫层析夹心法对2635例婴幼儿腹泻患儿新鲜粪便标本进行A群轮状病毒检测,并进行统计分析。结果 2635例标本中741例轮状病毒抗原检测阳性,总阳性率为28.12%,不同年龄段婴幼儿腹泻患儿轮状病毒感染率差异有统计学意义(P<0.05)。轮状病毒感染率在秋冬季节最高,春夏季节较低。在0～1岁年龄段中母乳喂养的婴儿抗轮状病毒能力与非母乳喂养者比较,差异有统计学意义(P<0.05)。结论轮状病毒是婴幼儿腹泻的主要病原体,及时进行轮状病毒抗原检测,对于诊断和合理预防治疗具有重大意义。

Objective To detect the group A rotavirus antigen in stool of 2 635 infants with diarrhea and provide rapid,accurate and reliable diagnostic basis for clinical practise.Methods Immunochromatographic sandwich method was employed to detect group A rotavirus antigen in fresh stool specimens of 2 635 infants with diarrhea and statisitical analysis was performed.Results In 2 635 specimens,741 were found rotavirus-positive,with total positive rate of 28.12%.The difference of rotavirus infection rates showed statistical significance among different ages of infants with diarrhea（P0.05）.The highest rotavirus infection rate was found in autumn and winter,and the lowest in spring and summer.Comparision between anti-rotavirus ability of breast-fed and non-breast-fed infants aged 0-1 year,the difference showed statistical significance（P0.05）.Conclusion Rotavirus is the major pathogen of infantile diarrhea.Timely rotavirus antigen detection has important significance in diagnosis and appropriate prevention and treatment.