小鼠海马发育过程中丝切蛋白及其磷酸化调节基因表达差异分析

Variance Expression Analysis of Cofilin and It Phosphorylation Regulation Genes During Mouse Hippocampus Development

论文旨在研究小鼠海马发育不同时期丝切蛋白及其磷酸化状态调节基因的表达量变化模式,为进一步研究丝切蛋白在海马发育过程中的功能以及其活性调节奠定基础。用半定量PCR检测不同时期丝切蛋白及其磷酸化调节基因相对表达量,通过ImageJ对结果进行分析,得出丝切蛋白及其磷酸化调节基因在小鼠海马发育不同时期表达量的变化趋势。结果表明,在小鼠海马发育不同时期丝切蛋白一直处于高表达状态,随着海马发育成熟丝切蛋白的表达量呈缓慢下降趋势。在这个过程中丝切蛋白磷酸化基因表达量呈明显下降趋势,P0时期LIMK1的表达量是P14时期的5.05倍(n=3)。与此相反,随着海马的发育中丝切蛋白去磷酸化基因表达量呈明显上升趋势,在P60时期CIN的表达量约是P0时期CIN表达量的2.88倍(n=3),P14时期SSH3的表达量约是P0时期的26.5倍(n=3)。其中LIMK2、TESK1、TESK2、SSH1和SSH2在这个过程中的表达量变化不明显。以上结果表明在小鼠海马发育过程中机体主要是通过调节丝切蛋白活性以满足不同发育时期对其活性的需求,其中LIMK1、CIN和SSH3是主要的丝切蛋白活性调节因子。

To lay the foundation of learning the function and it activity regulation of cofilin,we detected the changes of the expression of cofilin and it phosphorylation state regulatory genes during the development of mouse hippocampus.We used semiquantitative PCR to detect the relative quantity of cofilin and it phosphorylation regulatory genes in different stages.At the same time,ImageJ was used to analysis the results of the variation tendency of cofilin and it phosphorylation regulatory genes in different development stages.The results showed that the expression of cofilin is always high,it just slowly decreased with the development of hippocampus.However,the expression of cofilin phosphorylation genes decreases obviously,the expression level of LIMK1at P0is about 5.05（n=3）times when it compared to that at P14.On the contrary,the expression of Cofilin dephosphorylation genes shows a clear ascendant trend during this time.The expression level of CIN increased 2.88（n=3）times from P0to P60,at the same time SSH3increased 26.5（n=3）times.But the changes of LIMK2,TESK1,TESK2,SSH1and SSH2are not obviously.In conclusion,it mainly regulates the activity of cofilin to meet the demand of the development of mouse hippocampus.Among them,LIMK1,CIN and SSH3are the key factors to regulate the activity of cofilin.