彩色马铃薯块茎花色素苷分析测定

Determination and Analysis of Total Anthocyanin in Colorful Potatoes

彩色马铃薯是天然色素的潜在来源之一。本试验综合利用紫外/可见光谱法和pH示差法,初步定性和定量分析了6个彩色马铃薯品种块茎的花色素苷。结果表明,不同颜色马铃薯块茎花色素苷的类型和含量不同。紫色马铃薯师大6号和小乌洋芋的主要花色素苷为锦葵色素-3-葡萄糖苷,含量分别为1.086和0.376 mg/g.FW。红色品种的主要花色素苷为天竺葵色素-3-葡萄糖苷,含量为0.159～0.873 mg/g.FW。同一品种块茎不同组织的花色素苷含量存在明显差异。基于上述结果,对花色素苷的简易测定方法进行了简要的分析和讨论。

The colorful potato is one of the potential resources of natural colorants.The anthocyanin in six colorful potato cultivars/landraces were qualitatively and quantitatively analyzed by a combination of spectrophotometric method and pH differential method.The results showed that the type and content of anthocyanins was different among the different potato cultivars/landraces.Malvidin-3-glycoside was the major component of anthocyanins in the purple-fleshed cultivars including the Shida No.6 and the little black potato,which the amount was 1.086 and 0.376 mg/g·FW,respectively.Pelargonidin-3-glycoside was the major component of anthocyanins in the red-skin/fleshed cultivars/landraces,which the amount was ranged from 0.159 to 0.873 mg/g·FW.In addition,anthocyanin contents was significant difference in the different parts of one tuber.Based on the above results,a rapid and simple method for anthocyanin determination was discussed at the last.