黄颡鱼源温和气单胞菌对氟苯尼考耐药性获得与消失速率研究

Gaining and Disappearing Rates in Drug Resistance of Florfenicol with Aeromonas sobri by Yellow Catfish(Pelteobagrus fulvidraco)

在离体条件下,以36℃培养48 h为1代,测定5株黄颡鱼源温和气单胞菌(Aeromonas sobri)对氟苯尼考的耐药性获得与消失速率。结果显示,试验菌株在含有氟苯尼考的药物培养基中经连续传代诱导7代后,对氟苯尼考均获得了较高且稳定的耐药性,最低抑菌浓度(MIC)值分别上升了8～64倍;将已获得高耐药性的试验菌株接种在不含药物的营养肉汤培养基中连续传代至第5代后,MIC值呈稳定状态,但耐药性的消失速率很慢,其中As-004菌株的MIC值不变,其他4株菌株的MIC值也仅下降了50%。试验结果为水产养殖中科学选药和规范用药提供了理论依据,在生产实践中具有参考价值和指导意义。

The trial in vitro, cultivating 48h as one generation in 36℃, determined the gaining and disappearing rates in the drug resistance of florfenicol with Aeromonas sobri by Yellow Catfish （ Pelteobagrus fulvidraco）. The experimental result showed that these plants became highly and stably drug-fast to florfenieol in the culture medium of florfenicol after continuous passage and inducing 7 generations with the val- ue of MIC ascending to 8 - 64 times. After continuous passage and inducing 5 generations, the value of MIC presented stable state （ therein- to, the MIC value of As-O04 plant was invariable and the MIC value of other 4 plants decreased 50% only） and the drug resistance disap- peared slowly, as these plants were inoculated in culture medium without florfenicol. The result offers one thcoretical model to select prepcr drug and control drug in aquaculture, which has reference value and guidance in practice.