RNA酶(RNase)-胶体金法显示艾氏腹水癌细胞RNA的分布

Detection of RNA in Ehrlich ascite tumor cells by the RNase-Gold method

用RNase-胶体金法显示艾氏腹水癌细胞的RNA分布。在癌细胞核的颗粒区RNase-胶体金颗粒的分布比在纤维部密集,癌细胞质内的RNase-胶体金颗粒分布比较均匀。荷瘤小鼠腹腔连续4次注射大蒜乳剂后,多数艾氏腹水癌细胞的细胞核固缩,胞质内出现大量的空泡,癌细胞核及细胞质内的RNase-胶体金颗粒明显减少。结果表明RNase-胶体金标记是显示肿瘤细胞内RNA含量与分布的比较理想的半定量方法。也表明大蒜乳剂可能具有抑制艾氏腹水癌细胞RNA合成的作用。

The amount and distribution of RNA in Ehrlich ascite tumor cells were disp-lated by the RNAase-Gold method. The RNAase-Gold granules were much more in granular component than in the fibrillar component of the nucleous, The RNase-Gold granules in the cytoplasm were well-distributed. In the garlic emulsion treated group, the Ehrlich ascite tumor cells exhibed the plasmic vaculation and karyopy-knosis, and the amount of RNase-Gold granules in the nucleus and cytoplasm were reduced. The results showed that the RNase-Gold labeling was a method of semi-quantitating assessment of RNA in Ehrlich ascite tumor cells. The garlic emulsion had a effect of inhibiting RNA synthesis in Ehrlich ascite tumor cells.