哮喘患者血清IL-6,TNF-α,IL-10表达变化与气道炎症的关系探讨

Correlation between the levels of IL-6,TNF-α and IL-10 in serum and airway inflammation of patients with bronchial asthma

目的探讨哮喘患者血清中白细胞介素6和10(IL-6/10)以及血清肿瘤坏死因子-α(TNF-α)表达变化以及与气道炎症的关系。方法随机选择我院收治的哮喘患者100例和体检正常者50例,采用酶联免疫吸附法(ELISA)检测所有哮喘患者治疗前和后IL-6、IL-8及TNF-a、水平,同时与正常组比较。结果急性发作期和慢性持续期BA患者IL-6和TNF-α水平,均显著高于健康对照组(P<0.05),而IL-10水平显著低于健康组(P<0.05)。急性发作期BA患者IL-6和TNF-α水平,显著高于慢性续期组(P<0.05),而IL-10水平显著低于慢性持续组(P<0.05)。结论哮喘患者血清IL-6,TNF-α,IL-10的表达变化与病情的严重程度密切相关,3种细胞因子均有可能参与了哮喘的炎性反应。

Objective To investigate the correlation between the levels of IL-6,TNF-α and IL-10 in serum and airway inflammation of patients with bronchial asthma.Methods 100 patients with bronchial asthma and 50 healthy people were randomly selected in this study.The ELISA was used to detect and compare the levels of the IL-6,TNF-α and IL-10 in serum in all patients before and after the treatment.The logistic regression model was used to analyze the correlation between the levels of the IL-6,TNF-α and IL-10 and airway inflammation.Results The levels of IL-6 and TNF-α were the highest in the BA patients at acute exacerbation,followed by the BA patients at chronic persistent period and the healthy controls（P〈0.05）,while the level of IL-10 was significantly lowest in the BA patients at acute exacerbation（P〈0.05）.Conclusion There is a close correlation between the levels of IL-6,TNF-α and IL-10 and the severity of the bronchial asthma.The three kinds of cytokines may be involved in asthma inflammatory reaction.