Con A型差异糖蛋白在大鼠肝再生中的作用研究

Study of the role of differential Con A glycoprotein in rat liver regeneration

为研究差异糖蛋白在肝再生中的作用,运用Con A凝集素固相亲和层析、双向电泳和质谱等技术对大鼠再生肝的糖蛋白质组学进行研究,共鉴定出123种蛋白.有117种蛋白具有潜在的N-或O-糖基化位点;差异分析结果表明,共有33种糖蛋白在肝再生中发生有意义表达,其中,有20种蛋白发生上调表达,9种蛋白发生下调表达,4种蛋白发生上/下调表达.IPA分析表明这些差异蛋白主要参与p70S6K信号通路、PI3K/AKT通路,进一步分析发现NR1H4、GNMT、F16P、BGLR、H6PD、ALDH、14-3-3Z、ASSY、CYTB和ACTB与大鼠肝再生相关,这些糖蛋白均含有不同程度潜在的糖基化位点.本研究有助于进一步开展糖链结构解析和糖基化对蛋白功能的影响,以及糖基化蛋白在肝再生中的作用机理研究.

To explore the role of glycoprotein in rat liver regeneration, this study analyzes the glycoprotein differential expression profile during rat liver regeneration using Concanavalin-A affinity chromatography, 2D-PAGE and MALDI-TOF/TOF technology. The result shows that, among 123 proteins, there are 117 proteins which has potential N- or O- glycosylation site. Difference analysis indicates that, 33 proteins have differential expression during liver regeneration, the numbers of up-, down- and up/down- regulated proteins are 20, 9 and 4, respectively. Pathway analysis by IPA software shows that, these differential proteins mainly participate in p70S6K and PI3K/AKT signaling pathway which maybe regulate liver regeneration. Further analysis reveals that, the protein of NRIH4, GNMT, F16P, BGLR, H6PD, ALDH, 14-3-3Z, ASSY, CYTB and ACTB ave closely related to rat liver regeneration after partial hepatectomy. This research will contribute to study the glycopeptide＇s structure of glycoprotein and the bio-function of protein glycosylation, especially the role in liver regeneration