不同因子对黄秋葵毛状根生长的影响

Effects of different factors on the growth of hair roots in Abelmoschus esculentus L.Moench

目的探讨不同因子对黄秋葵毛状根生长的影响。方法利用增殖倍数法测定毛状根的生长,从而明确各因子对黄秋葵毛状根的影响。结果 3%蔗糖浓度最适宜毛状根的生长。0.5mg/L 6-BA对毛状根有一定的抑制作用,0.5mg/L NAA、IAA对毛状根有一定的促进作用,尤其是0.5mg/L IAA培养的毛状根增殖倍数更为显著。MJA对毛状根生长有抑制作用,SA对毛状根的生长没有明显的影响。结论不同因子不同程度地影响了毛状根的生长,为进一步筛选适宜的黄秋葵毛状根培养体系奠定了基础。

Objective： To explore the effects of different factors on the growth of hairy roots in Abelmoschus esculentus L.Moench.Methods： Method of proliferation multiple were used to measure the growth of hairy roots ,and definite the effects of different factors on the growth of hairy roots in A belmoschus esculentus L.Moench.Results： The most suitable sucrose concentration to the growth of hairy roots is 3%. 6-BA of O.5 mg.L-1 has a certain of inhibition to the hairy roots. The NAA and IAA of O.5 mg .L-1 has a certain promotion effect on the hairy roots, especially the multiplication is more notable for which the hairy roots cultivated in the IAA of O.5 mg＇L-1. MJA has a certain inhibition to the hairy roots and SA has no effect on the hairy roots.Conclusion： Different factors effect on the growth of hairy roots in different degrees and it lays the foundation for further screening the suitable cultivating sstem of the hairy roots of A belmoschus esculentus L.Moench.