CYP11B2基因-344C/T多态性与慢性心力衰竭及其醛固酮水平的相关性

Correlation of CYP11B2 gene 344C/T polymorphisms and chronic heart failure and aldosterone levels

目的探讨CYP11B2基因-344C/T多态性与慢性心力衰竭(CHF)及其醛固酮水平的相关性。方法选取CHF患者80例(CHF组)及健康体检者80例(健康对照组),以PCR-RFLP方法检测CYP11 B2基因-344C/T多态性,以ELISA法测定血浆醛固酮水平。结果 CHF组收缩压及舒张压明显高于健康对照组(P<0.05);2组基因型分布均符合Hardy-Weinberg平衡定律,CHF组CC、CT、TT基因型及等位基因C、T的分布频率分别为7.50%、41.25%、51.25%、29.38%、70.62%,健康对照组分别为13.75%、42.50%、43.75%、36.25%、63.75%,分布频率比较差异无统计学意义(P>0.05);CC、CT、TT基因型醛固酮水平差异具有统计学意义(P<0.05),CT和TT基因型醛固酮水平明显高于CC基因型(P<0.05)。结论 CYP11B2基因-344C/T多态性与慢性心力衰竭的发病无相关性,CYP1 1 B2基因-344T等位基因与慢性心力衰竭患者的醛固酮分泌水平之间具有相关性。

Objective To investigate the correlation of CYP11 B2 gene 344 C/T polymorphism and chronic heart failure （CHF） and aldosterone levels. Methods Selected 80 patients with CHF （ CHF group） and 80 cases of medical examination of normal people （healthy control group）, PCR-RFLP method in detecting CYP11 B2 gene 344 C/T polymorphism and plasma aldosterone levels with ELISA method. Results Systolic pressure and diastolic pressure in CHF group was obviously higher than that of healthy control group （ P 〈 0.05 ） ; Genotype distribution of two group were in line with the law of Hardy Weinberg equilibrium, CHF group of CC and CT, TF genotype and allele C and the T distribution frequency respectively 7.50% ,41.25% ,51.25% ,29.38% ,70. 62%, yheahh control group were 13.75%, 42.50% ,43.75%, 36.25% ,63.75%, distribution frequency comparison there was no statistically significant difference （ P 〉 0.05 ） ; CC, CT, TT genotype aldosterone level differences statistically significant （ P 〈 0.05 ）, CT and TT genotype aldosterone levels are significantly higher than CC genotype （ P 〈 0.05 ）. Conclusion CYP11 B2 gene 344 C/T polymorphism and the onset of chronic heart failure, there is no clear evidence of correlation, CYP11 B2 gene 344 T allele and patients with chronic heart failure correlation between levels of aldosterone secretion.