肿瘤坏死因子受体相关因子6在骨髓瘤细胞中的表达

The expression of TRAF6 in multiple myeloma cells and the relationship with myeloma markers

目的:观察多发性骨髓瘤细胞中TRAF6的表达水平及其与患者各项临床指标的关系。方法:采用RT-PCR及Western Blot技术检测骨髓瘤细胞株及患者骨髓单个核细胞中TRAF6的表达状况,并与患者外周血乳酸脱氢酶(LDH)、β2-微球蛋白(β2M)、白蛋白(ALB)表达水平作相关性分析。结果:(1)TRAF6在骨髓瘤细胞株及患者骨髓单个核细胞中的表达水平均高于正常对照组。(2)患者骨髓单个核细胞TRAF6蛋白含量与β2M表达成正相关(r=0.882,P<0.01),与ALB表达成负相关(r=-0.846,P<0.01),与LDH表达无相关(r=0.245,P>0.05)。结论:TRAF6在骨髓瘤细胞中表达增高,且与患者β2M、ALB的表达水平相关。

Objective：To determine the expression of TRAF6 in multiple myeloma（MM） cell lines and to observe the level of TRAF6 in bone marrow mononuclear cells of MM patients and the relationship between tumor markers in patients.Methods：By RT-PCR and Western Blot the expression of TRAF6 in multiple myeloma cell lines of KM3,U266 and RPMI8226 was detected.The expression of TRAF6 in bone marrow mononuclear cells of MM patients and controls was determined.The correlativity of the expressions of LDH,β2M and albumin was observed by testing peripheral blood and compared with the expression of TRAF6 in BM mononuclear cells.Results：（1）The levels of TRAF6 in myeloma cell lines and in myeloma BM mononuclear cells were higher than in the control group.（2）The levels of TRAF6 in myeloma BM mononuclear cells were positively correlated with its peripheral β2M（P0.01）,and negatively correlated with its peripheral albumin（P〈0.01）.But there was no significant correlation with LDH（P0.05）.Conclusion： The expression of TRAF6 in myeloma cells was higher than normal and was positively correlated with peripheral β2M,and negatively correlated with peripheral albumin.