脑栓通抑制凋亡并减轻脑梗死后丘脑继发性损害的研究

Naoshuantong inhibits apoptotic cell death and ameliorates secondary neuronal damage in the ipsilateral thalamus after focal cerebral infarction

目的 研究脑栓通对脑梗死后同侧丘脑继发性损害的神经保护作用及其可能机制。方法 采用高血压大鼠(RHRSP)建立一侧大脑中动脉皮层支闭塞(MCAO)模型,随机分为:假手术组、溶剂对照组和脑栓通组,每组8只。脑栓通组大鼠MCAO术后24h经灌胃给予2ml/kg(10mg/ml)脑栓通,溶剂对照组给予等剂量溶剂,连续6天。MCAO术后1周感觉功能评估后行尼氏染色评价脑梗死灶体积和丘脑损害,并行免疫组化检测丘脑TUNEL、MAP-2和GFAP表达。结果 脑栓通组大鼠患肢感觉功能较溶剂对照组出现明显改善(P<0.05)。两组间脑梗死灶体积无显著差异(P>0.05)。脑栓通治疗后同侧丘脑神经细胞数量、MAP-2表达较溶剂对照组显著增多(P均<0.05),但GFAP表达显著下降(P<0.05)。脑梗死后同侧丘脑TUNEL+细胞数量明显高于假手组,脑栓通可显著减少同侧丘脑TUNEL+细胞数量(P均<0.05)。结论 脑栓通能够改善脑梗死后同侧丘脑继发性神经损害和神经功能,其机制可能与抑制同侧丘脑细胞凋亡有关。

Objectives To explore the protective effects of Naoshuantong against the secondary damage and the underlying mechanism after focal cortical infarction in hypertensive rats.Materials and Methods Middle cerebral artery occlusion（MCAO） was induced in stroke-prone renovascular hypertensive rats and the operated rats were randomly divided into three groups：sham group,vehicle group and Naoshuantong group（8 rats per group）.One week after MCAO,the somatosensory deficits were tested.The neuronal damage in the ipsilateral thalamus was determined by Nissl staining or immunofluorescence analysis of MAP-2 and GFAP.Additionally,apoptosis was evaluated by TUNEL staining.Results Neurological score in Naoshuantong group was significantly lower than those in vehicle group（P〈0.05）.The infarct volumes were not significantly different between Naoshuantong and vehicle groups（P〈0.05）.Nissl staining showed that the number of intact neurons in the ipsilateral thalamus of Naoshuantong group was markedly higher than that in vehicle group（P〈0.05）.Consistently,Naoshuantong treatment obviously enhanced MAP-2 positive cells in the ipsilateral thalamus compared to vehicle group（P〈0.05）.Additionally,the number of GFAP positive cells in the ipsilateral thalamus was significantly reduced by Naoshuantong treatment（P〈0.05）.In parallel,the number of TUNEL positive cells in the ipsilateral thalamus in Naoshuantong group was significantly lower than that invehicle group（P〈0.05）.Conclusions Naoshuantong can reduce panoptic cell death and ameliorate the secondary damage of ipsilateral thalamus and neurological function following focal cerebral infarction.