石油烃降解混合菌修复稠油污染土壤的影响因素

Affecting Factors of Bioremediation in Improving Soil Contaminated by Thickened Oil

稠油在开采、贮运、炼制加工及使用过程中,不可避免的污染土壤环境,对人体健康和环境造成严重影响,因此有必要采取有效措施对稠油污染土壤进行修复。利用筛选获得的石油烃降解混合菌KL9-1,对稠油污染土壤进行修复研究,考察修复过程中的影响因素。结果表明:pH、接种量、土壤中N/P、表面活性剂用量、翻耕频率、浇水频率和膨松剂种类等因素对污染土壤修复有明显的影响。在每千克稠油污染土壤体系中,控制初始pH为8.0,接种量为70.0 mL,N∶P为3∶1,表面活性剂用量为4.0 g,每2 d浇水1次,4 d翻耕1次,稻壳作为膨松剂,在此条件下经过70 d的生物修复,石油烃降解率最高可达54.07%。

Thickened oil causes inevitably the serious contamination of edatope and impacts human health and environment in its mining,storage,transportation,refining and using process.It is necessary to take effective measures for improving soil contaminated by thickened oil.The microbial screened consortium KL9-1 was used to research the bioremediation for improving soil contaminated by thickened oil,and the factors in the biodegradation progress were studied.The results showed that the pH value,inoculum size,soil N/P,surfactant dosage,tillage frequency,watering frequency and raising agent affected significantly the bioremediation.In the soil contaminated by thickened oil per kilogram,the initial conditions of pH of 8.0,inoculum of 70.0 mL,N/P of 3∶ 1 and surfactant dosage of 4.0 g were controlled,the soil was watered every two days and plowed every four days,rice husks were used as a raising agent,and the petroleum hydrocarbon degradation rate could be as high as 54.07% after carrying out a biodegradation for 70 days.